小鼠Erythropoitin ELISA试剂盒(EPO) (ab119593)

概述

  • 产品名称
    小鼠Erythropoitin ELISA试剂盒(EPO)
    参阅全部 Erythropoietin (EPO) 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Tissue Homogenate
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    46.9 pg/ml - 3000 pg/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s mouse erythropoitin (EPO) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse erythropoitin (EPO) in cell culture supernatants, serum, plasma (heparin) and tissue homogenates. 

    A erythropoitin (EPO) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for erythropoitin (EPO) is added subsequently followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse erythropoitin (EPO) amount of sample captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab119593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119593

实验方案

文献

ab119593 has not yet been referenced specifically in any publications.

客户评价及客户问答

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ab119593: Erythropoietin

Good Excellent 5/5 (Ease of Use)
Abreviews
Sample: Mouse serum
Specification: EDTA treated mouse blood plasma
Erythropoietin levels were measured in mouse blood plasma samples collected during harvest in EDTA-containing syringes.
Samples were diluted 1:4.
The Erythropoietin mouse ELISA Kit works out fine and is easy to use.
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提交于 Apr 25 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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