小鼠Epiregulin ELISA试剂盒(EREG) (ab100682)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Epiregulin ELISA试剂盒(EREG)
参阅全部 Epiregulin 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
8.23 pg/ml - 6000 pg/ml -
回收率
83 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 102.6 94% - 110% Plasma 73.94 67% - 90% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse
不与反应: Human -
产品概述
Abcam’s Epiregulin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Epiregulin in plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Epiregulin coated on a 96- well plate. Standards and samples are pipetted into the wells and Epiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Epiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Epiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse Epiregulin in normal serum with ab100682, only in serum spiked with Mouse Epiregulin.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Epiregulin 2 vials Epiregulin Microplate (12 x 8 wells) 1 unit Recombinant Mouse Epiregulin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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相关性
Epiregulin is a member of the EGF-related growth factor family. It is a mitogen and has numerous roles including as an insulinotropic factor in pancreatic beta cell lines. It may also be involved in metastasis. -
别名
- EPR
- ER
- EREG
- Proepiregulin
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数据库链接
- SwissProt: Q61521 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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