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Signal Transduction Growth Factors/Hormones EGF

小鼠Epiregulin ELISA试剂盒(EREG) (ab100682)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)

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Typical Standard Curve
  • Typical Standard Curve

Key features and details

  • Sensitivity: 10 pg/ml
  • Range: 8.23 pg/ml - 6000 pg/ml
  • Sample type: Cell culture supernatant, Plasma
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

概述

  • 产品名称

    小鼠Epiregulin ELISA试剂盒(EREG)
    参阅全部 Epiregulin 试剂盒
  • 检测方法

    Colorimetric
  • 样品类型

    Cell culture supernatant, Plasma
  • 检测类型

    Sandwich (quantitative)
  • 灵敏度

    < 10 pg/ml
  • 范围

    8.23 pg/ml - 6000 pg/ml
  • 回收率

    83 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 102.6 94% - 110%
    Plasma 73.94 67% - 90%
  • 实验步骤

    Multiple steps standard assay
  • 种属反应性

    与反应: Mouse
    不与反应: Human
  • 产品概述

    Abcam’s Epiregulin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Epiregulin in plasma and cell culture supernatants.


    This assay employs an antibody specific for Mouse Epiregulin coated on a 96- well plate. Standards and samples are pipetted into the wells and Epiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Epiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Epiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Mouse Epiregulin in normal serum with ab100682, only in serum spiked with Mouse Epiregulin.

  • 平台

    Microplate

性能

  • 存放说明

    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse Epiregulin 2 vials
    Epiregulin Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse Epiregulin Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究领域

    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cancer
    • Growth factors
    • EGF
    • Cancer
    • Tumor biomarkers
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
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    • Tumor biomarkers ELISA kits
  • 相关性

    Epiregulin is a member of the EGF-related growth factor family. It is a mitogen and has numerous roles including as an insulinotropic factor in pancreatic beta cell lines. It may also be involved in metastasis.
  • 别名

    • EPR
    • ER
    • EREG
    • Proepiregulin
  • 数据库链接

    • SwissProt: Q61521 Mouse

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100682

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100682

    实验方案

    • Protocol Booklet

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab100682?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab100682 尚未被引用在任何文献中。

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question


    This is to confirm that I have received the proforma invoice. I will discuss this with my head of department and I will get back to you as soon as possible (the discounted price seems to fit to our budget but I really need to confirm this)
    As we have discussed on the phone, I am unsure about the Epiregulin kit since it has not yet been validated for use in human plasma. Maybe someone from Abcam's technical or research & development department could comment on this?
    Thank you in advance.
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2012

    Answer

    Thank you for contacting us.

    The Epiregulin kit ab100682 is not suitable for Human samples since one of the antibody has shown no cross-reactivity with Human epiregulin, and unfortunately we do not have an ELISA kit for Human Epiregulin in our catalogue.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Aug 10 2012

    Question

    Do the antibodies in this kit cross-react with human protein? EGF or amphiregulin?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 09 2012

    Answer

    Thank you very much for your call this week and for your patience while I have been in touch with the lab regarding your enquiry.

    The capture antibodyin this kit does cross-react with the human epiregulin, however the detection antibodyis not cross-reactive with the human protein. So, this kit will not be suitable for use with human samples, unfortunately. There is no cross reactivity with mouse or rat EGF or amphiregulin either.

    I hope that this information will be useful. I'm sorry that we do not have a human epiregulin kit at this time, but I would recommend searching on http://www.biocompare.com to see if another vendor carries one.

    Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    回复于 Aug 09 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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