小鼠DPPIV ELISA试剂盒(CD26) (ab155455)
Key features and details
- Sensitivity: 0.45 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠DPPIV ELISA试剂盒(CD26) -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.45 ng/ml -
范围
0.41 ng/ml - 100 ng/ml -
回收率
> 68 %
特定样本回收率 样品类型 平均% 范围 Serum 101.4 94% - 109% Plasma 88.06 77% - 97% Cell culture media 77.89 68% - 89% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam's DPPIV (CD26) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse DPPIV (CD26) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for DPPIV coated on a 96-well plate. Standards and samples are pipetted into the wells and DPPIV present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DPPIV antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DPPIV bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-mouse DPPIV 2 vials DPPIV Microplate (12 x 8 wells) 1 unit Recombinant Mouse DPPIV Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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数据库链接
- Entrez Gene: 13482 Mouse
- SwissProt: P28843 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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