Key features and details
- Sensitivity: 3 pg/ml
- Range: 2.741 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
参阅全部 CXCL9 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 3 pg/ml
范围2.741 pg/ml - 2000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 74.58 67% - 81% Serum 91.81 80% - 101% Plasma 85.55 77% - 93%
实验步骤Multiple steps standard assay
Abcam’s CXCL9 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CXCL9 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CXCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CXCL9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Mouse CXCL9 ELISA Kit (CXCL9) (ab203364) from our SimpleStep ELISA® range.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 240X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-mouse CXCL9 2 vials CXCL9 Microplate (12 x 8 wells) 1 unit Recombinant Mouse CXCL9 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
功能Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
- Information by UniProt
- C-X-C motif chemokine 9
- chemokine (C-X-C motif) ligand 9
SimpleStep ELISA kits
ab100725 被引用在 1 文献中.
- Yu J et al. Targeting macrophage priming by polyphyllin VII triggers anti-tumor immunity via STING-governed cytotoxic T-cell infiltration in lung cancer. Sci Rep 10:21360 (2020). PubMed: 33288772