小鼠Beta nerve growth factor ELISA试剂盒(ab277398)
Key features and details
- Sensitivity: 40 pg/ml
- Range: 0.041 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Beta nerve growth factor ELISA试剂盒
参阅全部 Beta nerve growth factor 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% General < 10% 批次间 样品 n Mean SD CV% General < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
40 pg/ml -
范围
0.041 ng/ml - 100 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 117.8 81% - 149% Plasma 108.8 78% - 148% Cell culture media 108.4 79% - 139% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse
预测可用于: Human -
产品概述
Mouse Beta nerve growth factor ELISA Kit (ab277398) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Beta nerve growth factor in serum (mouse beta-NGF concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Beta nerve growth factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse Beta nerve growth factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse Beta nerve growth factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse Beta nerve growth factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Mouse Beta nerve growth factor Antibody 2 vials Anti-Mouse Beta nerve growth factor coated Microplate 1 unit Mouse Beta nerve growth factor standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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相关性
Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta. -
细胞定位
Secreted. -
别名
- Beta nerve growth factor
- Beta NGF
- HSAN5
see all -
数据库链接
- Entrez Gene: 4803 Human
- Entrez Gene: 18049 Mouse
- Omim: 162030 Human
- SwissProt: P01138 Human
- SwissProt: P01139 Mouse
图片
数据表及文件
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SDS download
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Datasheet download
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