小鼠Angiopoietin 2 ELISA试剂盒(ab209883)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17.3 pg/ml
- Range: 68.75 pg/ml - 4400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Angiopoietin 2 ELISA试剂盒
参阅全部 Angiopoietin 2/ANG2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Mouse plasma 3 8.3% 批次间 样品 n Mean SD CV% Mouse plasma 5 10.3% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
17.3 pg/ml -
范围
68.75 pg/ml - 4400 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 94.5 67.4% - 120.9% Cell culture media 99.7 94.3% - 103.3% Hep Plasma 112.4 81.4% - 143.2% EDTA Plasma 104.9 91.8% - 122.8% Cit plasma 94.7 87.1% - 103.3% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse
不与反应: Cow -
产品概述
Mouse Angiopoietin 2 ELISA Kit (ab209883) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiopoietin 2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Angiopoietin 2 with 17.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Angiopoietin 2 is a secreted glycosylated protein functioning in angiogenesis, inflammation and vascular development. Angiopoietin 2 binds to receptor tyrosine kinase TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signalling. Angiopoietin 2 can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, Angiopoietin 2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, Angiopoietin 2 may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse Angiopoietin 2 Capture Antibody 1 x 600µl 10X Mouse Angiopoietin 2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse Angiopoietin 2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. -
序列相似性
Contains 1 fibrinogen C-terminal domain. -
结构域
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor. -
细胞定位
Secreted. - Information by UniProt
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别名
- AGPT 2
- Agpt2
- ANG 2
see all -
数据库链接
- Entrez Gene: 11601 Mouse
- SwissProt: O35608 Mouse
- Unigene: 439874 Mouse
相关产品
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Alternative Versions
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ELISA kits
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Related Products
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Angiopoietin 2 were measured in duplicates, interpolated from the Angiopoietin 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 6%, plasma (EDTA) 7.5%, plasma (heparin) 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin 2 concentration was determined to be 19,017 pg/mL in mouse serum, 2,802 pg/mL in rat serum, 31,192 pg/mL in mouse plasma (citrate), 26,451 pg/mL in mouse plasma (EDTA) and 33,857 pg/mL in mouse plasma (heparin).
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab209883 被引用在 3 文献中.
- Hultström M et al. Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients. Biomedicines 10:N/A (2022). PubMed: 35740360
- Meligy FY et al. Testing alternatives: the use of adipose-derived mesenchymal stem cells to slow neurodegeneration in a rat model of Parkinson's disease. Mol Biol Rep 46:5841-5858 (2019). PubMed: 31396803
- Lu W et al. Transplantation of Rat Mesenchymal Stem Cells Overexpressing Hypoxia-Inducible Factor 2aImproves Blood Perfusion and Arteriogenesis in a Rat Hindlimb Ischemia Model. Stem Cells Int 2017:3794817 (2017). PubMed: 29238372