小鼠and兔Specific HRP/DAB IHC Detection试剂盒- Micro-polymer (ab236466)
概述
-
产品名称
小鼠and兔Specific HRP/DAB IHC Detection试剂盒- Micro-polymer
参阅全部 HRP/DAB detection kit 试剂盒 -
检测方法
Colorimetric -
检测类型
Micro-Polymer -
产品概述
This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.
-
说明
The previous version of this kit, the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 150,000 slides since 2012 (based on volumes of reagent sold).
Other IHC detection products
For alternative kits, see anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469, and HRP-AEC micro-polymer IHC kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
-
经测试应用
适用于: IHC-Pmore details
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 15 ml 60 ml 125 ml 50x DAB Chromogen 1 x 0.5ml 1 x 2ml 1 x 4ml DAB substrate 1 x 15ml 1 x 60ml 1 x 125ml Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml 1 x 125ml Hydrogen Peroxide Block 1 x 15ml 1 x 60ml 1 x 125ml Mouse Specifying Reagent (Complement) 1 x 15ml 1 x 60ml 1 x 125ml Protein block 1 x 15ml 1 x 60ml 1 x 125ml -
研究领域
-
相关性
HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab236466于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
说明 |
---|
IHC-P
Use at an assay dependent concentration. |
图片
-
Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the skin. (A) The upper panel shows representative MMP-2 staining of skin from CTRL and MFS mice at 12 months of age with or without DOX, and the lower panel shows bar graphs representing the expression of MMP-2 in the skin of CTRL and MFS mice at 12 weeks of age with or without DOX. (B) The upper panel shows representative MMP-9 staining of skin from CTRL and MFS mice at 12 months of age with or without DOX treatment. The lower panel shows bar graphs representing the expression of MMP-9 in the skin of CTRL and MFS mice at 12 weeks of age treated with or without DOX.
-
Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the aorta.Immunohistochemistry staining images and bar graphs representing the expression of (A) MMP-2 and (B) MMP-9 in the aortic roots of CTRL and MFS mice at 12 weeks of age treated with or without DOX.
-
ab236466 Mouse and Rabbit Specific HRP-DAB Detection IHC Kit on Human Tonsil staining CD20
-
ab209064 at 1:2000 staining TMEM119 antibody in mouse cerebrum tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TMEM119 with ab209064 at 1/2000 dilution followed by the same HRP micro-polymer goat anti-rabbit secondary antibody used in this kit. Positive staining on glial cells in mouse cerebrum is observed. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. Counter stained with hematoxylin.
-
ab1220 staining human kidney sections by IHC-P using EXPOSE IHC detection kit ab80436 (this kit is identical to ab80436, except that it uses the same HRP polymer secondary antibody used by Abcam's R&D team for antibody validation). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH 6) for 30 minutes. The section was incubated with ab1220, 5 µg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.
数据表及文件
-
SDS download
-
Datasheet download
文献 (47)
ab236466 被引用在 47 文献中.
- Trevelin SC et al. Disrupted Peyer's Patch Microanatomy in COVID-19 Including Germinal Centre Atrophy Independent of Local Virus. Front Immunol 13:838328 (2022). PubMed: 35251032
- Novotny T et al. The possible role of hypoxia in the affected tissue of relapsed clubfoot. Sci Rep 12:4462 (2022). PubMed: 35292718
- Efimova EV et al. Trace Amine-Associated Receptor 2 Is Expressed in the Limbic Brain Areas and Is Involved in Dopamine Regulation and Adult Neurogenesis. Front Behav Neurosci 16:847410 (2022). PubMed: 35431833
- Sadamoto S et al. Comparison Approach for Identifying Missed Invasive Fungal Infections in Formalin-Fixed, Paraffin-Embedded Autopsy Specimens. J Fungi (Basel) 8:N/A (2022). PubMed: 35448568
- Schregel J et al. Effects of oral glutamine supplementation on jejunal morphology, development, and amino acid profiles in male low birth weight suckling piglets. PLoS One 17:e0267357 (2022). PubMed: 35476806