小鼠ACE ELISA试剂盒(CD143) (ab155452)
Key features and details
- Sensitivity: 0.12 ng/ml
- Range: 0.123 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠ACE ELISA试剂盒(CD143)
参阅全部 Angiotensin Converting Enzyme 1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% overall < 10% 批次间 样品 n Mean SD CV% overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.12 ng/ml -
范围
0.123 ng/ml - 30 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 104.6 96% - 112% Plasma 101.4 94% - 109% Cell culture media 99.78 90% - 109% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam's ACE (CD143) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse ACE (CD143) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse ACE coated on a 96-well plate. Standards and samples are pipetted into the wells and ACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse ACE antibody is added. After washing away unbound biotinylated antibody,
HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl ACE Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated anti-mouse ACE 2 vials Recombinant Mouse ACE Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 8ml -
研究领域
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功能
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. -
组织特异性
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. -
疾病相关
Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. -
序列相似性
Belongs to the peptidase M2 family. -
翻译后修饰
Phosphorylated by CK2 on Ser-1299; which allows membrane retention. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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别名
- ACE
- ACE 1
- ACE T
see all -
数据库链接
- Entrez Gene: 11421 Mouse
- SwissProt: P09470 Mouse
- Unigene: 754 Mouse
图片
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab155452 被引用在 2 文献中.
- Shen L et al. Effects of 1,25-dihydroxyvitamin D3 on the local bone renin-angiotensin system in a murine model of glucocorticoid-induced osteoporosis. Exp Ther Med 13:3297-3304 (2017). PubMed: 28587403
- Zheng J et al. Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats. Mol Med Rep 16:8485-8492 (2017). PubMed: 28944898