Anti-Monoacylglycerol Lipase/MGL抗体(ab228598)
Key features and details
- Rabbit polyclonal to Monoacylglycerol Lipase/MGL
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Monoacylglycerol Lipase/MGL抗体
参阅全部 Monoacylglycerol Lipase/MGL 一抗 -
描述
兔多克隆抗体to Monoacylglycerol Lipase/MGL -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Pig -
免疫原
Recombinant fragment within Human Monoacylglycerol Lipase/MGL (internal sequence). The exact sequence is proprietary.
Database link: Q99685 -
阳性对照
- WB: Mouse white adipose tissue lysate; HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Mouse esophagus tissue; rat fore brain tissue; U-373 xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab228598于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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说明 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
靶标
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功能
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. -
组织特异性
Detected in adipose tissue, lung, liver, kidney, brain and heart. -
通路
Glycerolipid metabolism; triacylglycerol degradation. -
序列相似性
Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family. - Information by UniProt
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数据库链接
- Entrez Gene: 505290 Cow
- Entrez Gene: 11343 Human
- Entrez Gene: 23945 Mouse
- Entrez Gene: 29254 Rat
- Omim: 609699 Human
- SwissProt: Q99685 Human
- SwissProt: O35678 Mouse
- SwissProt: Q8R431 Rat
see all -
别名
- EC 3.1.1.23 antibody
- HU K5 antibody
- HU-K5 antibody
see all
图片
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Paraffin-embedded mouse esophagus tissue stained for Monoacylglycerol Lipase/MGL with ab228598 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-Monoacylglycerol Lipase/MGL antibody (ab228598) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 33 kDa10% SDS-PAGE
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Paraffin-embedded rat forebrain tissue stained for Monoacylglycerol Lipase/MGL with ab228598 at 1/500 dilution in immunohistochemical analysis.
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Anti-Monoacylglycerol Lipase/MGL antibody (ab228598) at 1/1000 dilution + Mouse white adipose tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 33 kDa10% SDS-PAGE
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Paraffin-embedded U-373 (human) xenograft tissue stained for Monoacylglycerol Lipase/MGL with ab228598 at 1/500 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab228598 被引用在 1 文献中.
- Wang C et al. Inhibition of monoacylglycerol lipase restrains proliferation, migration, invasion, tumor growth and induces apoptosis in cervical cancer. J Obstet Gynaecol Res 48:456-466 (2022). PubMed: 34877750