Anti-mono methyl Arginine抗体[5D1] (ab415)

Submit an Abreview Q&A (3)Specific References (3)

概述

  • 产品名称
    Anti-mono methyl Arginine抗体[5D1]
    参阅全部 mono methyl Arginine 一抗
  • 描述
    小鼠单克隆抗体[5D1] to mono methyl Arginine
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, ChIPmore details
  • 种属反应性
    与反应: Species independent
  • 常规说明

    This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.

性能

应用

Our Abpromise guarantee covers the use of ab415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
ChIP Use 5µl for 106 cells.

靶标

  • 别名
    • Arginine (mono methyl) antibody

图片

  • ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)
    ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)This image is courtesy of Sylvain Daujat, Tony Kouzarides lab, Cambridge University, UK

    Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab415 to mono methyl Arginine.

    Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5 µl of ab415 and 4x106 cells were used in each ChIP experiment.

  • ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)
    ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)

    We looked at chicken chromatin and then analysed 4 active genes (AG1-4), 2 inactive genes (IG1-2) as well as heterochromatin (Het). A "Yes" indicates that the antibody immunoprecipitated material well above background and should be regarded as a positive result.

     

文献

This product has been referenced in:
  • Slade DJ  et al. Chemical and biological methods to detect post-translational modifications of arginine. Biopolymers 101:133-43 (2014). Read more (PubMed: 23576281) »
  • Raijmakers R  et al. Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro. J Mol Biol 367:1118-29 (2007). Read more (PubMed: 17303166) »

See all 3 Publications for this product

发表研究结果有使用 ab415？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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3 reviews or Q&A

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Votes: Highest First

samples; HEK293 cells block: 5% milk ab413, ab415: black membrane, protein ladder in white Ab 1/1000 suggestion: use less primary and/or secondary antibody ab109451: lots of bands, Ab 1/1000 suggestion: use less primary ...

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Thank you for your phone call. I am glad to hear that for ab109451 the protocol tips did work. I am very sorry that unfortunately, for ab413 and ab415, the tips did not improve your results. However, the details provided enable us to closely monitor th...

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samples; HEK293 cells block: 5% milk ab413, ab415: black membrane, protein ladder in white Ab 1/1000 suggestion: use less primary and/or secondary antibody ab109451: lots of bands, Ab 1/1000 suggestion: use less primary antibody (recommended ...

Read More

Thank you for your phone call. I am sorry to hear that these 3 antibodies are not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality....

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Concerning ab415, ab414, ab413 - says whole antiserum; is it tisue culture supernatant or ascites? Do ab412 and ab413 cross-reat with symmetrical Ng/Na - dimethyl arginine?

Thank you for your patience. Ab415, ab414, and ab413 are ascites. It has not been determinded as to whether ab412 and ab413 cross-react with symmetrical Ng/Na - dimethyl arginine.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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