Key features and details
- Mouse monoclonal [5D1] to mono methyl Arginine
- Suitable for: WB, ChIP
- Reacts with: Species independent
- Isotype: IgG1
产品名称Anti-mono methyl Arginine抗体[5D1]
参阅全部 mono methyl Arginine 一抗
描述小鼠单克隆抗体[5D1] to mono methyl Arginine
经测试应用适用于: WB, ChIPmore details
种属反应性与反应: Species independent
This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
Concentration information loading...
纯度Protein A purified
Primary antibody说明This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.
ChIP Related Products
Our Abpromise guarantee covers the use of ab415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
|ChIP||Use 5µl for 106 cells.|
- Arginine (mono methyl) antibody
Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab415 to mono methyl Arginine.
Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5
µl of ab415 and 4x106 cells were used in each ChIP experiment.
We looked at chicken chromatin and then analysed 4 active genes (AG1-4), 2 inactive genes (IG1-2) as well as heterochromatin (Het). A "Yes" indicates that the antibody immunoprecipitated material well above background and should be regarded as a positive result.
ab415 被引用在 4 文献中.
- Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell 173:1385-1397.e14 (2018). PubMed: 29706550
- Slade DJ et al. Chemical and biological methods to detect post-translational modifications of arginine. Biopolymers 101:133-43 (2014). PubMed: 23576281
- Raijmakers R et al. Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro. J Mol Biol 367:1118-29 (2007). PubMed: 17303166
- Cuthbert GL et al. Histone deimination antagonizes arginine methylation. Cell 118:545-53 (2004). PubMed: 15339660