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Epigenetics and Nuclear Signaling Histones H3 Methylated

Anti-mono methyl Arginine抗体[5D1] (ab415)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (4)

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ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)
  • ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)

Key features and details

  • Mouse monoclonal [5D1] to mono methyl Arginine
  • Suitable for: WB, ChIP
  • Reacts with: Species independent
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-mono methyl Arginine抗体[5D1]
    参阅全部 mono methyl Arginine 一抗
  • 描述

    小鼠单克隆抗体[5D1] to mono methyl Arginine
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, ChIPmore details
  • 种属反应性

    与反应: Species independent
  • 常规说明

    This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • 纯度

    Protein A purified
  • Primary antibody说明

    This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.
  • 克隆

    单克隆
  • 克隆编号

    5D1
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Epigenetics and Nuclear Signaling
    • Histones
    • H2A
    • Methylated
    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Methylated
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
Use at an assay dependent dilution.
ChIP
Use 5µl for 106 cells.
说明
WB
Use at an assay dependent dilution.
ChIP
Use 5µl for 106 cells.

靶标

  • 别名

    • Arginine (mono methyl) antibody

图片

  • ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)
    ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)This image is courtesy of Sylvain Daujat, Tony Kouzarides lab, Cambridge University, UK

    Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab415 to mono methyl Arginine.

    Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5 µl of ab415 and 4x106 cells were used in each ChIP experiment.

  • ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)
    ChIP - Anti-mono methyl Arginine antibody [5D1] (ab415)

    We looked at chicken chromatin and then analysed 4 active genes (AG1-4), 2 inactive genes (IG1-2) as well as heterochromatin (Het). A "Yes" indicates that the antibody immunoprecipitated material well above background and should be regarded as a positive result.

     

实验方案

  • ChIP protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (4)

发表研究结果有使用 ab415?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab415 被引用在 4 文献中.

  • Cheung P  et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell 173:1385-1397.e14 (2018). PubMed: 29706550
  • Slade DJ  et al. Chemical and biological methods to detect post-translational modifications of arginine. Biopolymers 101:133-43 (2014). PubMed: 23576281
  • Raijmakers R  et al. Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro. J Mol Biol 367:1118-29 (2007). PubMed: 17303166
  • Cuthbert GL  et al. Histone deimination antagonizes arginine methylation. Cell 118:545-53 (2004). PubMed: 15339660

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

samples; HEK293 cells block: 5% milk ab413, ab415: black membrane, protein ladder in white Ab 1/1000 suggestion: use less primary and/or secondary antibody ab109451: lots of bands, Ab 1/1000 suggestion: use less primary antibody (recommended dilution is 1/10,000 to 1/50,000)

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Abcam community

Verified customer

Asked on Dec 05 2011

Answer

Thank you for your phone call. I am glad to hear that for ab109451 the protocol tips did work. I am very sorry that unfortunately, for ab413 and ab415, the tips did not improve your results. However, the details provided enable us to closely monitor the quality of our products. I am sorry these 2 products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements with the order number xxx for ab107495 and ab107514. You should receive both vials tomorrow. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Abcam Scientific Support

回复于 Dec 05 2011

Question

samples; HEK293 cells block: 5% milk ab413, ab415: black membrane, protein ladder in white Ab 1/1000 suggestion: use less primary and/or secondary antibody ab109451: lots of bands, Ab 1/1000 suggestion: use less primary antibody (recommended dilution is 1/10,000 to 1/50,000)

Read More

Abcam community

Verified customer

Asked on Nov 22 2011

Answer

Thank you for your phone call. I am sorry to hear that these 3 antibodies are not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. As we discussed over the phone, I would like to offer some suggestions to help optimize the results: For ab413, ab415 I'd suggest using less primary and/or secondary antibody as the membrane shouldn't turn black. This might be an indication of too much antibody on the membrane. For ab109451, most likely using less primary antibody could help with the background as the recommended dilution for the antibody is 1/10,000 to 1/50,000. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

Read More

Abcam Scientific Support

回复于 Nov 22 2011

Question

Concerning ab415, ab414, ab413 - says whole antiserum; is it tisue culture supernatant or ascites? Do ab412 and ab413 cross-reat with symmetrical Ng/Na - dimethyl arginine?

Read More

Abcam community

Verified customer

Asked on Apr 13 2004

Answer

Thank you for your patience. Ab415, ab414, and ab413 are ascites. It has not been determinded as to whether ab412 and ab413 cross-react with symmetrical Ng/Na - dimethyl arginine.

Read More

Abcam Scientific Support

回复于 Apr 28 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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