Anti-mono and dimethyl Arginine抗体[7E6] (ab412)


  • 产品名称
    Anti-mono and dimethyl Arginine抗体[7E6]
  • 描述
    小鼠单克隆抗体[7E6] to mono and dimethyl Arginine
  • 宿主
  • 经测试应用
    适用于: IP, ChIP, ELISAmore details
    不适用于: WB
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Asymmetrical NG/NG-dimethyl arginine

  • 阳性对照
    • Methylated histones are an option
  • 常规说明

    This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises both dimethyl and monomethyl arginine it can be used in parallel with ab413 (detects dimethyl arginine only) and ab414 (detects monomethyl arginine only) to monitor the exact modification status. A tissue culture supernatant version is available as ab5394.



Our Abpromise guarantee covers the use of ab412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
ChIP Use 3µl for 106 cells.
ELISA Use a concentration of 0.05 - 0.5 µg/ml.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 别名
      • Di methyl Arginine antibody
      • Dimethyl Arginine antibody
      • Methyl Arginine antibody
      • Mono methyl Arginine antibody
      • Monomethyl Arginine antibody
      see all


    • Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab412 to mono and dimethyl Arginine.

      Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 3 µl of ab412 and 4x106 cells were used in each ChIP experiment.

    • RIP140 null adipocytes extracts were suspended in 250 µl of immunoprecipitation buffer (150 mM NaCl,50 mM Tris-HCl (pH 8.0), 1 mM EDTA, 0.2% (v/v) Nonidet P40, 2 mM PMSF, 0.1% (w/v) SDS and a protease-inhibitor cocktail. Protein extracts (200 µg) were incubated with antibodies to RIP140, 14-3-3, or PRMT1 overnight at 4°C, and precipitated with protein G–agarose beads for 1–2 hours. ab412 was then used (amongst other antibodies) in Western Blot. Reduced protein inputs were used for ectopic expression of RIP140 to avoid saturation.


    This product has been referenced in:
    • Ganesan M  et al. Demethylase JMJD6 as a New Regulator of Interferon Signaling: Effects of HCV and Ethanol Metabolism. Cell Mol Gastroenterol Hepatol 5:101-112 (2018). Read more (PubMed: 29693039) »
    • Kong GM  et al. Selective small-chemical inhibitors of protein arginine methyltransferase 5 with anti-lung cancer activity. PLoS One 12:e0181601 (2017). Read more (PubMed: 28806746) »

    See all 31 Publications for this product


    Immuno-precipitation step
    Protein A/G
    Human Cell lysate - whole cell (fibroblast)
    Total protein in input
    1000 µg

    Abcam user community

    Verified customer

    提交于 Jun 19 2014

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