重组Anti-MMP8抗体[EP1252Y] - Low endotoxin,Azide free (ab224265)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1252Y] to MMP8 - Low endotoxin, Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MMP8抗体[EP1252Y] - Low endotoxin,Azide free
参阅全部 MMP8 一抗 -
描述
兔单克隆抗体[EP1252Y] to MMP8 - Low endotoxin,Azide free -
宿主
Rabbit -
特异性
WB positive for Human recombinant MMP8 but negative results for the following cell lines and tissue: HeLa, A431,K562 and bone marrow.
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经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Rat lung lysate. IHC-P: Human urinary bladder carcinoma and placenta tissues. IP: Rat lung lysate.
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常规说明
ab224265 is the carrier-free version of ab81286.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1252Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224265于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Can degrade fibrillar type I, II, and III collagens. -
组织特异性
Neutrophils. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
细胞定位
Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules. - Information by UniProt
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数据库链接
- Entrez Gene: 4317 Human
- Entrez Gene: 17394 Mouse
- Entrez Gene: 63849 Rat
- Omim: 120355 Human
- SwissProt: P22894 Human
- SwissProt: O70138 Mouse
- SwissProt: O88766 Rat
- Unigene: 161839 Human
see all -
别名
- CLG 1 antibody
- CLG1 antibody
- Collagenase 1 antibody
see all
图片
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ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Purified ab81286 at 1/50 dilution (2µg) immunoprecipitating MMP8 in Rat lung lysate.
Lane 1 (input): Rat lung lysate 10µg
Lane 2 (+): ab81286 + Rat lung lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab81286 in Rat lung lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 70 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81286). -
This IHC data was generated using the same anti-MMP8 antibody clone [EP1252Y] in a different buffer formulation (cat# ab81286).
ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (10)
ab224265 被引用在 10 文献中.
- McKay TB et al. Acute hypoxia influences collagen and matrix metalloproteinase expression by human keratoconus cells in vitro. PLoS One 12:e0176017 (2017). WB ; Human . PubMed: 28426715
- Xu M et al. Tumor Necrosis Factor-Like Weak Inducer of Apoptosis Promotes Hepatic Stellate Cells Migration via Canonical NF-?B/MMP9 Pathway. PLoS One 11:e0167658 (2016). WB ; Human . PubMed: 27907201
- Luo K et al. Association between postmenopausal osteoporosis and experimental periodontitis. Biomed Res Int 2014:316134 (2014). IHC-P ; Rat . PubMed: 24683547
- Villalta PC et al. Role of MMP2 and MMP9 in TRPV4-induced lung injury. Am J Physiol Lung Cell Mol Physiol 307:L652-9 (2014). PubMed: 25150065
- Thirkettle S et al. Matrix metalloproteinase 8 (collagenase 2) induces the expression of interleukins 6 and 8 in breast cancer cells. J Biol Chem 288:16282-94 (2013). WB ; Human . PubMed: 23632023
- Bradley JM et al. Exposure to diesel exhaust particulates induces cardiac dysfunction and remodeling. J Appl Physiol (1985) 115:1099-106 (2013). WB ; Rat . PubMed: 23887904
- Misra Y et al. Mammary gland morphological and gene expression changes underlying pregnancy protection of breast cancer tumorigenesis. Physiol Genomics 44:76-88 (2012). PubMed: 22085904
- Chiao YA et al. Matrix metalloproteinase-9 deletion attenuates myocardial fibrosis and diastolic dysfunction in ageing mice. Cardiovasc Res : (2012). WB . PubMed: 22918978
- Loo AE et al. Effects of hydrogen peroxide on wound healing in mice in relation to oxidative damage. PLoS One 7:e49215 (2012). WB ; Mouse . PubMed: 23152875
- Holt R et al. The molecular mechanisms of cervical ripening differ between term and preterm birth. Endocrinology 152:1036-46 (2011). PubMed: 21209014