重组
RabMAb

Anti-MMP3抗体[EP1186Y] (ab52915)

概述

  • 产品名称
    Anti-MMP3抗体[EP1186Y]
    参阅全部 MMP3 一抗
  • 描述
    兔单克隆抗体[EP1186Y] to MMP3
  • 宿主
    Rabbit
  • 特异性
    79% identities with MMP10
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human MMP3 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • Human gastric carcinoma tissue.
  • 常规说明

     

    A trial size is available to purchase for this antibody.

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

应用

Our Abpromise guarantee covers the use of ab52915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/50.
ICC/IF 1/100 - 1/250.

靶标

  • 功能
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 序列相似性
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CHDS6 antibody
    • Matrix metalloproteinase 3 antibody
    • Matrix metalloproteinase-3 antibody
    • MGC126102 antibody
    • MGC126103 antibody
    • MGC126104 antibody
    • MMP 3 antibody
    • MMP-3 antibody
    • MMP3 antibody
    • MMP3_HUMAN antibody
    • Proteoglycanase antibody
    • SL-1 antibody
    • SL1 antibody
    • STMY antibody
    • STMY1 antibody
    • STR1 antibody
    • Stromelysin 1 antibody
    • Stromelysin-1 antibody
    • Transin 1 antibody
    • Transin-1 antibody
    see all

图片

  • Immunohistochemical staining of paraffin embedded rat kidney with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunofluorescence staining of HT-29 cells with purified ab52915 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52915 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte )whole cell lysate
    Lane 2 : HEK293 (Human embryonic kidney epithelial cell ) whole cell lysate
    Lane 3 : Mouse lung lysate
    Lane 4 : Mouse placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)


    Exposure time: 84 seconds


    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)

    Lane 1 : Rat brain lysate
    Lane 2 : Rat liver lysate
    Lane 3 : Rat spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)



    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 & 2: 10 seconds
                             Lane 3: 3 minutes


  • All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time:  Lane 1: 3 minutes
                               
    Lane 2 & 3: 4 seconds

  • Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified) + Rat kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)


    Exposure time: 15 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

  • Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/20000 dilution (purified) + MMP3 recombinant protein

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded mouse liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

文献

This product has been referenced in:
  • Jeon J  et al. 3'-Sialyllactose protects against osteoarthritic development by facilitating cartilage homeostasis. J Cell Mol Med 22:57-66 (2018). WB . Read more (PubMed: 28782172) »
  • Liu X  et al. Protective effect of curcumin against ultraviolet A irradiation-induced photoaging in human dermal fibroblasts. Mol Med Rep 17:7227-7237 (2018). Read more (PubMed: 29568864) »

See all 38 Publications for this product

客户评价及客户问答

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This product will detect total MMP3.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

The MSDS can be downloaded from website. It is now linked to the datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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