Anti-MMP2抗体[4D3] (ab2462)

Reviews (3)Q&A (11)References (24)
Western blot - Anti-MMP2 antibody [4D3] (ab2462)
  • Western blot - Anti-MMP2 antibody [4D3] (ab2462)

Key features and details

  • Mouse monoclonal [4D3] to MMP2
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-MMP2抗体[4D3]
    参阅全部 MMP2 一抗

  • 描述

    小鼠单克隆抗体[4D3] to MMP2

  • 宿主

    Mouse

  • 特异性

    Binds to human MMP-2 and pro-MMP2. The antibody was tested for cross-reactivity with MMP-1, MMP-3, and MMP-9 and showed mild cross-reactivity with MMP-3.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide corresponding to Human MMP2 aa 557-569 (internal sequence).
    Sequence:

    TSLGLPPDVQRVD


    Database link: P08253
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • Colon carcinoma. Placental tissue

性能

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
WB
Human
All applications
Mouse
Rat
应用 Ab评论 说明
WB (1)
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration.

靶标

  • 功能

    Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.

  • 组织特异性

    Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.

  • 疾病相关

    Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.

  • 序列相似性

    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.

  • 结构域

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • 翻译后修饰

    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.

  • 细胞定位

    Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 72 kDa gelatinase antibody
    • 72kD type IV collagenase antibody
    • CLG 4 antibody
    • CLG 4A antibody
    • CLG4 antibody
    • CLG4A antibody
    • Collagenase Type 4 alpha antibody
    • Collagenase type IV A antibody
    • Gelatinase A antibody
    • Gelatinase alpha antibody
    • Gelatinase neutrophil antibody
    • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
    • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 2 antibody
    • Matrix metalloproteinase II antibody
    • Matrix metalloproteinase-2 antibody
    • MMP 2 antibody
    • MMP II antibody
    • MMP-2 antibody
    • MMP2 antibody
    • MMP2_HUMAN antibody
    • MONA antibody
    • Neutrophil gelatinase antibody
    • PEX antibody
    • TBE 1 antibody
    • TBE-1 antibody
    see all

图片

  • Western blot - Anti-MMP2 antibody [4D3] (ab2462)
    Western blot - Anti-MMP2 antibody [4D3] (ab2462)

    400 ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane, and detected by immunoblotting with ab2462 specific for MMP-2.

  • Western blot - Anti-MMP2 antibody [4D3] (ab2462)
    Western blot - Anti-MMP2 antibody [4D3] (ab2462)This image is courtesy of an anonymous Abreview
    Anti-MMP2 antibody [4D3] (ab2462) at 1/5000 dilution + Rabbit aorta tissue lysate at 40 µg

    Secondary
    HRP-conjugate Sheep anti-mouse IgG polyclonal

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 72 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds

    See Abreview

文献 (24)

发表研究结果有使用 ab2462？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2462 被引用在 24 文献中.

  • Xie H  et al. ROS/NF-?B Signaling Pathway-Mediated Transcriptional Activation of TRIM37 Promotes HBV-Associated Hepatic Fibrosis. Mol Ther Nucleic Acids 22:114-123 (2020). PubMed: 32916597
  • He C  et al. CircLMNB1 promotes colorectal cancer by regulating cell proliferation, apoptosis and epithelial-mesenchymal transition. Onco Targets Ther 12:6349-6359 (2019). PubMed: 31496737
  • Du J  et al. The novel circular RNA circ-CAMK2A enhances lung adenocarcinoma metastasis by regulating the miR-615-5p/fibronectin 1 pathway. Cell Mol Biol Lett 24:72 (2019). PubMed: 31889960
  • Zhu A  et al. Long non-coding RNA H19 down-regulates miR-181a to facilitate endothelial angiogenic function. Artif Cells Nanomed Biotechnol 47:2698-2705 (2019). PubMed: 31267802
  • Tian Y  et al. A reproducible swine model of proximal descending thoracic aortic aneurysm created with intra-adventitial application of elastase. J Vasc Surg 67:300-308.e2 (2018). PubMed: 28479097
View all Publications for this product

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Western blot abreview for Anti-MMP2 antibody [4D3]

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rabbit Tissue lysate - whole (aorta)
Specification
aorta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
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提交于 Jun 24 2013

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