MMP14抑制剂Screening Assay试剂盒(Colorimetric) (ab139454)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Inhibitor compounds
概述
-
产品名称
MMP14抑制剂Screening Assay试剂盒(Colorimetric)
参阅全部 MMP14 试剂盒 -
检测方法
Colorimetric -
样品类型
Inhibitor compounds -
检测类型
Enzyme activity -
产品概述
Abcam MMP14 Inhibitor Screening Assay Kit (Colorimetric) (ab139454) is a complete assay system designed to screen MMP14 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5’-dithiobis(2-nitrobenzoic acid), Ellman’s reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (ε=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format.
-
说明
This kit is useful to screen inhibitors of MMP14, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.
Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.
-
平台
Microplate reader
性能
-
存放说明
Please refer to protocols. -
组件 1 x 96 tests 96-well Clear Microplate (1/2 Volume) 1 unit Colorimetric Assay Buffer 1 x 20ml MMP Inhibitor 1 x 50µl MMP Substrate 1 x 50µl MMP14 Enzyme (Human, Recombinant) 1 x 25µl -
研究领域
-
功能
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. -
组织特异性
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
别名
- Matrix metallopeptidase 14 (membrane inserted)
- Matrix metalloproteinase 14
- Matrix metalloproteinase-14
see all
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab139454 尚未被引用在任何文献中。