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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
使用敲除细胞株进行验证重组RabMAb

重组Anti-MMP14抗体[EP1264Y] (ab51074)

  • Datasheet
  • SDS
Reviews (20)Q&A (8)References (134)

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Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Immunoprecipitation - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Flow Cytometry (Intracellular) - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Anti-MMP14 antibody [EP1264Y] (ab51074)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1264Y] to MMP14
  • Suitable for: IHC-P, IP, Flow Cyt (Intra), WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free HRP PE

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概述

  • 产品名称

    Anti-MMP14抗体[EP1264Y]
    参阅全部 MMP14 一抗
  • 描述

    兔单克隆抗体[EP1264Y] to MMP14
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, IP, Flow Cyt (Intra), WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab185123)

  • 阳性对照

    • IHC-P: Human endometrium carcinoma and kidney tissues. WB: Human fetal spleen, esophagus and lung cancer tissue lysates, mouse and rat spleen tissue lysates; A431 wild-type cell lysate and Caco-2 whole cell lysate. Flow Cyt (intra): HT-1080 cells. IP: A431 cell lysate. ICC/IF: HT-1080 cells and wild-type A431 cells.
  • 常规说明

    The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EP1264Y
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • ECM enzymes
    • MMPs
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

相关产品

  • Alternative Versions

    • Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)
    • HRP Anti-MMP14 antibody [EP1264Y] (ab194242)
    • Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)
  • Assay kits

    • MMP14 Inhibitor Screening Assay Kit (Colorimetric) (ab139454)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Immunizing Peptide (Blocking)

    • MMP14 peptide (ab185123)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human MMP14 knockout A-431 cell line (ab261890)
  • KO cell lysates

    • Human MMP14 knockout A-431 cell lysate (ab261699)
  • Positive Controls

    • A-431 whole cell lysate (ab30132)
  • Recombinant Protein

    • Recombinant human MMP14 protein (Active) (ab157068)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab51074于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (4)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/20.

For unpurified use at 1/100.

Flow Cyt (Intra)
1/20.
WB (8)
1/5000. Predicted molecular weight: 66 kDa.Can be blocked with MMP14 peptide (ab185123).

For unpurified use at 1/2000.

ICC/IF (6)
Use a concentration of 0.2 µg/ml.
说明
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/20.

For unpurified use at 1/100.

Flow Cyt (Intra)
1/20.
WB
1/5000. Predicted molecular weight: 66 kDa.Can be blocked with MMP14 peptide (ab185123).

For unpurified use at 1/2000.

ICC/IF
Use a concentration of 0.2 µg/ml.

靶标

  • 功能

    Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • 组织特异性

    Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • 序列相似性

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰

    The precursor is cleaved by a furin endopeptidase.
  • 细胞定位

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P50281 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 281915 Cow
    • Entrez Gene: 4323 Human
    • Entrez Gene: 17387 Mouse
    • Entrez Gene: 81707 Rat
    • Omim: 600754 Human
    • SwissProt: Q9GLE4 Cow
    • SwissProt: P50281 Human
    • SwissProt: P53690 Mouse
    • SwissProt: Q10739 Rat
    • Unigene: 2399 Human
    • Unigene: 280175 Mouse
    • Unigene: 10371 Rat
    see all
  • 别名

    • Matrix metallopeptidase 14 (membrane inserted) antibody
    • Matrix metalloproteinase 14 antibody
    • Matrix metalloproteinase-14 antibody
    • Membrane type 1 matrix metalloproteinase antibody
    • Membrane type 1 metalloprotease antibody
    • Membrane type matrix metalloproteinase 1 antibody
    • Membrane-type matrix metalloproteinase 1 antibody
    • Membrane-type-1 matrix metalloproteinase antibody
    • MMP 14 antibody
    • MMP X1 antibody
    • MMP-14 antibody
    • MMP-X1 antibody
    • Mmp14 antibody
    • MMP14_HUMAN antibody
    • MMPX1 antibody
    • MT MMP 1 antibody
    • MT-MMP 1 antibody
    • MT1 MMP antibody
    • MT1-MMP antibody
    • MT1MMP antibody
    • MTMMP 1 antibody
    • MTMMP1 antibody
    see all

图片

  • Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    All lanes : Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/2000 dilution

    Lane 1 : A431 wild-type cell lysate
    Lane 2 : A431 MMP14 knockout cell lysate
    Lane 3 : Caco-2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab51074 observed at 54 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab51074 was shown to react with MMP14 in A431 wild-type cells in Western blot. Loss of signal was observed when MMP14 knockout sample was used. A431 wild-type and MMP14 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% Milk in TBS-T (0.1% Tween®) before incubation with ab51074 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] (ab51074)
    ab51074 staining MMP14 in wild-type A431 cells (top panel) and MMP14 knockout A431 cells (bottom panel) (ab261890). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab51074 at 0.2μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
  • Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    All lanes : Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/2000 dilution (unpurified)

    Lane 1 : Human fetal spleen tissue lysate
    Lane 2 : Human lung cancer tissue lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 66 kDa
    Observed band size: 60,63 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    63 kDa: pro-form; 60 kDa: active form.

    MCF7 is a MMP14 negative or weakly expressed cell line (PMID: 25977338 and PMID: 19208838).

  • Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    All lanes : Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/5000 dilution (purified)

    Lane 1 : Human fetal spleen lysate
    Lane 2 : Human lung cancer lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : Human esophagus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)

    Immunohistochemical analysis of paraffin-embedded Human endometrial cancer labeling MMP14 with ab51074 at 1/500 (3.26 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on the human endometrial tumour stroma cells and weak staining on the tumour cells. The section was incubated with ab51074 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 


    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)

    Immunohistochemical analysis of paraffin-embedded Human endometrial cancer labeling MMP14 with ab51074 at 1/500 (3.26 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human endometrial cancer. The section was incubated with ab51074 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium carcinoma tissue sections labeling MMP14 with purified ab51074 at 1/100 dilution (1.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counterstain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution.

    PBS instead of the primary antibody was used as the negative control (inset).

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] (ab51074)

    ab51074 staining MMP14 in HT-1080 (human fibrosarcoma epithelial cell) cells by ICC/IF (Immunocytochemistry/Immunofluorescence).

    Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 1/1000 dilution (0.2 μg/ml). An Alexa Fluor® 488 Goat anti-rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, ab195889) was used as the counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic and weakly membranous staining in HT-1080 cell line.

    Negative control (bottom panels): MCF7 PMID: 19208838.

  • Immunoprecipitation - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Immunoprecipitation - Anti-MMP14 antibody [EP1264Y] (ab51074)

    ab51074 (purified) at 1/20 dilution (2 µg) immunoprecipitating MMP14 in A431 (human epidermoid carcinoma) whole cell lysate.

    Lane 1: A431 whole cell lysate 10ug
    Lane 2: ab51074 + A431 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab51074 in A431 whole cell lysate

    For western blotting, ab131366 VeriBlot for IP (HRP) was used for detection (1/1000).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Flow Cytometry (Intracellular) - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Flow Cytometry (Intracellular) - Anti-MMP14 antibody [EP1264Y] (ab51074)

    Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) / HT-1080 (Human fibrosarcoma epithelial cell, Right) cells labeling MMP14 with ab51074 at 1/200 dilution (0.1 µg) (red). Goat anti-rabbit IgG (Alexa Fluorr® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Rabbit monoclonal IgG (ab172730) / black was used as the isotype control. Cells incubated with secondary antibody only (blue) was used as the unlabeled control. Gated on viable cells. 

    Positive control (Right panel): HT-1080 cells. 

    Negative control (Left panel): MCF7 cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)This image is courtesy of an anonymous Abreview

    ab51074 (unpurified) at 1/500 staining human kidney tissue sections by IHC-P.

    The tissue was formaldehyde fixed and a heat mediated antigen retrieval step (in Tris/EDTA) was performed. The tissue was then blocked with serum and incubated with the primary antibody. A biotinylated donkey anti-rabbit IgG was used as the secondary.

    See Abreview

  • Anti-MMP14 antibody [EP1264Y] (ab51074)
    Anti-MMP14 antibody [EP1264Y] (ab51074)

实验方案

  • IHC-P protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (134)

发表研究结果有使用 ab51074?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab51074 被引用在 134 文献中.

  • Matsuzaki T  et al. Reversion-inducing cysteine-rich protein with Kazal motifs and MT1-MMP promote the formation of robust fibrillin fibers. J Cell Physiol 236:1980-1995 (2021). PubMed: 32730638
  • Alabi A  et al. Membrane type 1 matrix metalloproteinase promotes LDL receptor shedding and accelerates the development of atherosclerosis. Nat Commun 12:1889 (2021). PubMed: 33767172
  • Sántha P  et al. Morphological Heterogeneity in Pancreatic Cancer Reflects Structural and Functional Divergence. Cancers (Basel) 13:N/A (2021). PubMed: 33672734
  • Zhu Y  et al. Nogo-B promotes epithelial-mesenchymal transition in lung fibrosis via PERK branch of the endoplasmic reticulum stress pathway. Ann Transl Med 9:563 (2021). PubMed: 33987261
  • Mukenhirn M  et al. Role of Clathrin Light Chains in Regulating Invadopodia Formation. Cells 10:N/A (2021). PubMed: 33672612
View all Publications for this product

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1-10 of 20 Abreviews

Western blot abreview for Anti-MMP14 antibody [EP1264Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MC3T3 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
MC3T3 cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 04 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-MMP14 antibody [EP1264Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 11 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-MMP14 antibody [EP1264Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse vascular smooth muscle cells)
Permeabilization
Yes - NP40
Specification
Mouse vascular smooth muscle cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 11 2021

Western blot abreview for Anti-MMP14 antibody [EP1264Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (hiPSC-Brain Microvascular Endothelial Cells)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
20 µg
Specification
hiPSC-Brain Microvascular Endothelial Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Alessandra Granata

Verified customer

提交于 Dec 30 2019

Western blot abreview for Anti-MMP14 antibody [EP1264Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
15 µg
Specification
Melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 19 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MMP14 antibody [EP1264Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (vertebrae)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: 10 mg/mL hyaluronidase+100mU/mL chondrotinase ABC
Permeabilization
No
Specification
vertebrae
Blocking step
(agent) for 10 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative
10% NBF
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 14 2019

Western blot abreview for Anti-MMP14 antibody [EP1264Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE-19)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Loading amount
10 µg
Treatment
125 ug/ml cigarette smoke extract for 24hrs
Specification
ARPE-19
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 13 2019

Western blot abreview for Anti-MMP14 antibody [EP1264Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Vascular smooth muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 10 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-MMP14 antibody [EP1264Y]

Good
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain)
Permeabilization
Yes - 0.1% Triton-X in PBS
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Daniel Lustberg

Verified customer

提交于 May 25 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MMP14 antibody [EP1264Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (Airway)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Permeabilization
No
Specification
Airway
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 23 2015

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