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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP

Anti-MMP14抗体(ab3644)

  • Datasheet
  • SDS
Reviews (1)Q&A (6)References (19)

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Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody (ab3644)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (ab3644)

Key features and details

  • Rabbit polyclonal to MMP14
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-MMP14抗体
    参阅全部 MMP14 一抗
  • 描述

    兔多克隆抗体to MMP14
  • 宿主

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide within Human MMP14 aa 150-250. The exact sequence is proprietary.
    Database link: P50281

  • 常规说明

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • ECM enzymes
    • MMPs
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Assay kits

    • MMP14 Inhibitor Screening Assay Kit (Colorimetric) (ab139454)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human MMP14 protein (Active) (ab157068)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC/IF
Human
IHC-P
Human
应用 Ab评论 说明
IHC-P
1/30.
ICC/IF
Use a concentration of 1 µg/ml.
说明
IHC-P
1/30.
ICC/IF
Use a concentration of 1 µg/ml.

靶标

  • 功能

    Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • 组织特异性

    Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • 序列相似性

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰

    The precursor is cleaved by a furin endopeptidase.
  • 细胞定位

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P50281 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4323 Human
    • Omim: 600754 Human
    • SwissProt: P50281 Human
    • Unigene: 2399 Human
    • 别名

      • Matrix metallopeptidase 14 (membrane inserted) antibody
      • Matrix metalloproteinase 14 antibody
      • Matrix metalloproteinase-14 antibody
      • Membrane type 1 matrix metalloproteinase antibody
      • Membrane type 1 metalloprotease antibody
      • Membrane type matrix metalloproteinase 1 antibody
      • Membrane-type matrix metalloproteinase 1 antibody
      • Membrane-type-1 matrix metalloproteinase antibody
      • MMP 14 antibody
      • MMP X1 antibody
      • MMP-14 antibody
      • MMP-X1 antibody
      • Mmp14 antibody
      • MMP14_HUMAN antibody
      • MMPX1 antibody
      • MT MMP 1 antibody
      • MT-MMP 1 antibody
      • MT1 MMP antibody
      • MT1-MMP antibody
      • MT1MMP antibody
      • MTMMP 1 antibody
      • MTMMP1 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody (ab3644)
      Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody (ab3644)

      ICC/IF image of ab3644 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3644, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (ab3644)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (ab3644)

      IHC image of ab3644 staining in Human Colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3644, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (19)

    发表研究结果有使用 ab3644?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3644 被引用在 19 文献中.

    • Lin Z  et al. The effect of overexpression of the HOXD10 gene on the malignant proliferation, invasion, and tumor formation of pancreatic cancer cell PANC-1. Gland Surg 9:385-391 (2020). PubMed: 32420263
    • Lucken-Ardjomande Häsler S  et al. GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ?F508. J Cell Biol 219:N/A (2020). PubMed: 32344433
    • Lu H  et al. APE1 Upregulates MMP-14 via Redox-Sensitive ARF6-Mediated Recycling to Promote Cell Invasion of Esophageal Adenocarcinoma. Cancer Res 79:4426-4438 (2019). PubMed: 31308045
    • Ogawa K  et al. Aspartate ß-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway. J Hematol Oncol 12:144 (2019). PubMed: 31888763
    • Brodzikowska A  et al. Metalloproteinase 14 (MMP-14) and hsa-miR-410-3p expression in human inflamed dental pulp and odontoblasts. Histochem Cell Biol 152:345-353 (2019). PubMed: 31486923
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Question

    WB with human cell lysates (breast cancer)
    pos. crtl: no band at 66 kDa
    sees bands at 120 and 170 kDa
    reducing/denaturing
    Ab: 1/250, 1/2000
    2nd: works well
    block: 5% milk, BSA, superblock

    Read More

    Abcam community

    Verified customer

    Asked on Aug 16 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab51074.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Aug 16 2012

    Question

    Please provide the exact location or at least a range within which the immunogen is found in this protein.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 02 2007

    Answer

    Following-up my message from Friday, I did receive more information regarding ab3644: the immunogen is a 12 amino acid peptide found between amino acids 100-200 of human MPP-14. Since the pro-peptide is aa 1-111, and the antibody recognizes the active as well as latent forms of the protein, we can narrow this range further to aa 112-200. I hope this helps. For ab16267, I am afraid there is no more information available.

    Read More

    Abcam Scientific Support

    回复于 Apr 02 2007

    Question

    The gels that we have been using are non-reducing, non-denaturing. The amount of protein loaded vary according to our daily plans.... but should be more than sufficient in order to detect bands. We haven't used loading controls at this point... we wanted to see something first. We used the NP-40 buffer documented in your western blot beginners pdf file. In the case of all the antibodies ordered, they have been designated by Abcam as tested for Western blots. Do you have any images on files that I can take a look at? What type of tissue did you use for the testing? How different was your protocol from the one established in our lab? Sorry for all the questions but maybe I can use this info for some of the trouble shooting on our end.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 25 2006

    Answer

    Thank you for your enquiry. I appreciate your continued patience in this matter. I have received some feedback from some of the sources of the antibodies that you are enquiring about; ab7033 - To follow on with the comments that I have made the source of mouse monoclonal [MMP2/2C1] to MMP2 (ab7033) was also concerned with regards the approach that you have used for your sample preparation. I appreciate that you have been using an NP40 buffer extraction. However, this is an approach designed for a cytoplasmic preparation of cell culture cells. I would like to follow up my previous email by recommending that you perform a loading control experiment using an antibody that targets a "housekeeping protein" for example GAPDH or beta actin. This can be performed under the conditions best recognized by the antibody you are using; most likely denaturing, reduced. This will enable you to fully determine the integrity of your protein with respect to its protein composition. My biggest concern with the blot images that you have provided me with are the band doublet that you have been detecting either side of the 37KDa marker as this is present in the majority of your blot images. The source of ab6586 - Collagen IV antibody (ab6586) makes a similar suggestion although Collagens should in fact be electrophoresed as you have been using non-denaturing, non-reduced conditions. However, it is important that the integrity of your samples are confirmed. I am still awaiting further information and am in the process of requesting blot images for the antibodies that you have enquired about. I appreciate your continued patience. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Aug 01 2006

    Question

    Hi again. I submitted the form for ab 2500 (Laminin), however, I am having difficulty re-accessing the questionnaire for the remaining antibodies. For this reason, I will provide the necessary information in this email. I have not been satisfied with the following antibodies: ab11572, EMMPRIN: (bands not at proper place, band at approx. 37 kDa): I have already submitted images of this. ab7033, MMP2: (bands not at proper place, bands below 40 kDa): I have already submitted images of this. ab6586, Collagen IV: (no bands): I have already submitted images of this. 2 hours transfer period. Questions regarding: ab3644, MT1-MMP: bands appear at 50 kDa and approximately 40 kDa. MW should be 66 and 54 kDa. What were your results? Images previously sent. ab1828, TIMP2: bands appear at 25 and 37 kDa. Band is documented to appear at 21 kDa. What are your experiences with this? Images previously sent. For all antibodies: Please find protocol attached to this email. Gels used: Criterion precast (Biorad), non-reducing, percentage determined according to molecular weight investigated All buffers and secondary antibodies used were suggested by Abcam on the data sheets. All protocols included preliminary testing in either 3% skim milk and 3% BSA. If there is anymore information that I may supply, please do not hesitate to contact me. Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 20 2006

    Answer

    Thank you for your enquiry. Once again I am sorry to hear of the difficulties that you have experienced using these antibodies. I have had a look through the information that you have provided in conjunction with the blot images and I have a few comments. Firstly I am concerned about the sample preparation that has been performed. Many of the extraneous bands that have been detected are significantly lower than I would expect. Curiously many are also doublets at approximately 25KDa and 37KDa. My suspicion would be non-specific labeling possibly due to protein degradation. You have highlighted that the samples were prepared as recommended by Abcam. Please can you detail exactly how this was performed as we make many recommendations for sample preparation. My concern with respect to the sample preparation is that many of the proteins that your are targeting are secreted proteins and therefore require delicate sample preparation. Can you tell me whether the skeletal muscle samples were postmortum, biopsies, surgical specimens etc. I would also appreciate details of the gel conditions that you are using. Similar to my previous response with respect to ab2500 I would appreciate it if you could provide me with details of the gel conditions. I would like to know whether these pre-cast gels are non-denaturing in addition to non-reduced. I would also like details of how the samples are prepared immediately prior to loading on the gel. Can you also provide me with details of the primary and secondary antibody dilutions that have been attempted for each of the samples. Given the non-specific reactivity that you have observed I am also interested whether any no-antibody control experiments have been performed to determine whether the extraneous bands are attricubatble to this reactivity. I additionally have a few comments with respect to individual antibodies that you have been applying: In the experiments that have been performed using ab1828, the secondary that has been employed is ab6721. This is an antibody that is specific for use by IHC and has not been tested for use in western blotting. We have received some excellent feedback as to the application of ab3644 through our Abreviews system. I would like to highlight the importance of a a suitable positive control. For MT1-MMP/MMP14 we recommend the use of placental or breast/lung tissue. For Collagen IV we recommend the use of human epidermal keratinocytes. I look forward to your comments on these matters.

    Read More

    Abcam Scientific Support

    回复于 Jul 21 2006

    Question

    Please find attached images/comments about our optimization for western blots using your products. We have not been able to produce satisfactory results with the following antibodies: ab2500, ab6586, ab7033, ab1157 We also have questions pertaining to the following antibodies: ab1828, ab3644. We are happy to receive advice from past experiences that Abcam may have had in the process of testing these antibodies and are open to continuing the optimization process. However, if satisfactory results are not obtained, we will count on the Abcam product guarantee.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 22 2006

    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with these antibodies. The results that you have been obtaining are certainly troubling. What concerns me the most are the two bands that you are consistently detecting at approximately 25 and 37KDa. You are consistently detecing cross reacting bands and never detecting the endogenous target. Whilst some of the antibodies that you detail have not been tested using rat samples; potentially explaining the absence of reactivity with the endogenous band the presence of the two bands at the aforementioned molecular weight accross many of your samples seems to suggest problems with the secondary antibody. Especially in view of the fact that you have changed this antibody and are in fact using three independent secondary antibodies: Goat polyclonal to Rabbit IgG H&L (HRP) (ab6721) Goat polyclonal to Rabbit IgG + Mouse IgG & IgM - prediluted (HRP polymer) (ab2891) Rabbit polyclonal to Goat IgG H&L (HRP) (ab6741) I would appreciate it if you could provide me with protocol details of the approach that you are using so that I can better determine potential reasons for these reacting bands. We have not observed such reactivity with any of the antibodies you list. I am particularly interested in the generic protocol that you have been applying. To provide me with these details please click on the link below and complete our on line technical questionaire. If you have modified the protocol for any of the antibodies at any point please mention this. I am also very interested in the method of sample preparation and the control antibody (loading control) that you have used. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=2500&mode=questionaire I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    回复于 Jun 28 2006

    Western blot abreview for Anti-MMP14 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (cell lines: breast / lung)
    Loading amount
    15 µg
    Specification
    cell lines: breast / lung
    Blocking step
    Other as blocking agent for 30 minute(s) · Concentration: 0.05 % Twe
    Read More

    Abcam user community

    Verified customer

    提交于 May 04 2006

    Question

    A couple of questions from our Taiwan distributor: 1) What is the recommended dilution for ab10800? 2) What are the recommended positive controls for ab13018, ab7747, ab3644

    Read More

    Abcam community

    Verified customer

    Asked on Jul 28 2005

    Answer

    ab10800 -- Mouse monoclonal [HAS-3] to Integrin alpha 2 is provided at a concentration of 1mg/ml (this information was previosuly missing from the datasheet but has been added now). You do not mention which application the researcher plans to use the product, but knowing the concentration should now enable them to determine a starting dilution. There are also references on the datasheet that specifically feature the use of this antibody so please ask them to check here for further information. Positive controls: ab3644 -- Rabbit polyclonal to MMP14 Suitable tissue control sections for Immunohistochemistry: Placenta. Note the antibody cross reacts with human. This information is found on the positive control and cross reactivity sections of the datasheet. ab13018 -- Rabbit polyclonal to IL8 Receptor alpha There are beautiful IHC images on the datasheet showing staining of human skin, eccrine sweat glands. ab7747 -- Rabbit polyclonal to IL8 Shown to cross reacts with Human, Cynomolgus Monkey and Rhesus monkey. Product specific reference included on the datasheet is Fujii A et al. Differential expression of chemokines, chemokine receptors, cytokines and cytokine receptors in diffuse large B cell malignant lymphoma. Int J Oncol 24:529-38 (2004). PubMed: 14767537.

    Read More

    Abcam Scientific Support

    回复于 Jul 29 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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