Anti-MMP13抗体[M33] (ab1010)

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Key features and details

  • Mouse monoclonal [M33] to MMP13
  • Suitable for: WB, ELISA
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-MMP13抗体[M33]
    参阅全部 MMP13 一抗

  • 描述

    小鼠单克隆抗体[M33] to MMP13

  • 宿主

    Mouse

  • 特异性

    Based on Western blotting, the antibody reacts with human procollagenase-3. The antibody does not cross react with MT1-MMP latent form (MMP-14), MT2-MMP catalytic domain (MMP-15), MT3-MMP catalytic domain (MMP-16), MT4-MMP catalytic domain (MMP-17), MT5-MMP catalytic domain (MMP-18), Gelatinase A (MMP-2) latent form, Gelatinase B (MMP-9) latent form or Collagenase 2 (MMP-8) latent form.

  • 经测试应用

    适用于: WB, ELISAmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant human procollagenase-3 expressed in insect cells

  • 常规说明

    MMP is Matrix Metalloproteinase.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab1010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa).
ELISA 1/2000.

靶标

  • 功能

    Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.

  • 组织特异性

    Seems to be specific to breast carcinomas.

  • 疾病相关

    Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.

  • 序列相似性

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.

  • 结构域

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • 细胞定位

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CLG 3 antibody
      • CLG3 antibody
      • Collagenase 3 antibody
      • Collagenase3 antibody
      • MANDP1 antibody
      • Matrix metallopeptidase 13 (collagenase 3) antibody
      • Matrix Metalloproteinase 13 antibody
      • Matrix metalloproteinase-13 antibody
      • MMP 13 antibody
      • MMP-13 antibody
      • Mmp13 antibody
      • MMP13_HUMAN antibody
      see all

    文献 (0)

    发表研究结果有使用 ab1010？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab1010 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    Which MMP13 epitope is recognized by this monoclonal antibody?

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    Answer

    My sincere apologies for the delay in replying to you, we have tried to find out if the epitope has been mapped but I can confirm that it has not been mapped and therefore we do not know which portion of MMP13 is recognized by the antibody. Sorry I couldn't help you more,

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