MMP Activity Assay试剂盒(Fluorometric - Red) (ab112147)
Key features and details
- Assay type: Direct
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Purified protein, Tissue Lysate
概述
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产品名称
MMP Activity Assay试剂盒(Fluorometric - Red)
参阅全部 MMP 试剂盒 -
检测方法
Fluorescent -
样品类型
Purified protein, Tissue Lysate -
检测类型
Direct -
种属反应性
与反应: Mammals, Other species -
产品概述
MMP Activity Assay Kit (Fluorometric Red) ab112147 uses a fluorescence resonance energy transfer (FRET) peptide as a MMP substrate. In the intact FRET peptide, the fluorescence of one part is quenched by the other. Upon cleavage into two separate fragments by MMPs, the fluorescence is recovered.
The MMP assy is designed to check the general activity of a MMP enzyme in a tissue sample. It can also be used to screen MMP inhibitors when a purified MMP enzyme is used.
With excellent fluorescence quantum yield and longer wavelength, the substrate used in this assay shows less interference from autofluorescence of test compounds and cellular components and is much more sensitive than an EDANS/Dabcyl FRET substrate.
The MMP assay signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm. The pH-independent fluorescence makes the assay reading available for the whole physiological pH range.
The high photostability of this FRET peptide provides a useful imaging probe. Many labs have used this kit for the high throughput screening of MMP inhibitors as potential anticancer drug candidates. This assay might be also used for monitoring cancer cells.
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说明
ab112147 should be stored Desiccated
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests APMA, 4-Aminophenylmercuric Acetate 1 x 20µl Assay Buffer 1 x 20ml MMP Red Substrate 1 x 60µl -
研究领域
图片
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Tissues were lysed with RIPA buffer and activated with 2 mM APMA (1:1) for 1,2 and 3 hours at 37°C. Samples were then diluted to 5 mg/mL and 10 mg/mL with Assay Buffer. 50 µl of MMP containing sample was mixed with MMP Red Substrate. The fluorescence signal was monitored 30 min after the start of the reaction by using a microplate reader with a filter set of Ex/Em = 540/595 nm. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Red Substrate but no MMPs.
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Tissues were lysed with RIPA buffer and activated with 2 mM APMA (1:1) for 1,2 and 3 hours at 37°C. Samples were then diluted to 5 mg/mL and 10 mg/mL with Assay Buffer. 50 µl of MMP containing sample was mixed with MMP Red Substrate. The fluorescence signal was monitored 30 min after the start of the reaction by using a microplate reader with a filter set of Ex/Em = 540/595 nm. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Red Substrate but no MMPs.
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MMPs were activated with 2 mM APMA (1:1). Samples were then diluted to 0.6 µg/mL (30 ng per well) with Assay Buffer. 50 µl of MMP containing sample was mixed with MMP Red Substrate. The fluorescence signal was monitored 30 min after the start of the reaction by using a microplate reader with a filter set of Ex/Em = 540/595 nm. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Red Substrate but no MMPs.
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MMPs were activated with 2 mM APMA (1:1). Samples were then diluted to 0.6 µg/mL (30 ng per well) with Assay Buffer. 50 µl of MMP containing sample was mixed with MMP Red Substrate. The fluorescence signal was monitored 30 min after the start of the reaction by using a microplate reader with a filter set of Ex/Em = 540/595 nm. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Red Substrate but no MMPs.
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab112147 被引用在 14 文献中.
- Moos M et al. Cryoprotective Metabolites Are Sourced from Both External Diet and Internal Macromolecular Reserves during Metabolic Reprogramming for Freeze Tolerance in Drosophilid Fly, Chymomyza costata. Metabolites 12:N/A (2022). PubMed: 35208237
- Szabo A et al. A human iPSC-astroglia neurodevelopmental model reveals divergent transcriptomic patterns in schizophrenia. Transl Psychiatry 11:554 (2021). PubMed: 34716291
- Malakpour-Permlid A et al. Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds. Sci Rep 11:6655 (2021). PubMed: 33758206
- Boada C et al. Rapamycin-Loaded Biomimetic Nanoparticles Reverse Vascular Inflammation. Circ Res 126:25-37 (2020). PubMed: 31647755
- Shendi D et al. Hyaluronic acid as a macromolecular crowding agent for production of cell-derived matrices. Acta Biomater 100:292-305 (2019). PubMed: 31568877