重组Anti-MLKL (phospho S345)抗体[EPR9515(2)] - BSA and Azide free (ab208910)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345) - BSA and Azide free
- Suitable for: WB, IP, Dot blot
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-MLKL (phospho S345)抗体[EPR9515(2)] - BSA and Azide free
参阅全部 MLKL 一抗 -
描述
兔单克隆抗体[EPR9515(2)] to MLKL (phospho S345) - BSA and Azide free -
宿主
Rabbit -
特异性
MLKL pS345 is a trigger for necroptosis. It is only detectable in infection/cellular damaged (PMID:29229989) or aging tissue (PMID: 28807105) but not in normal tissues. -
经测试应用
适用于: WB, IP, Dot blotmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: L929 whole cell lysate treated with 20 ng/ml TNF alpha, 100 nM Smac mimetic, and 20 mM z-VAD for 8h and then harvested. IP: L929 whole cell lysate treated with TNF alpha+ Smac mimetic + z-VAD compound for 8h.
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常规说明
ab208910 is the carrier-free version of ab196436.
This antibody was developed through collaboration with the lab of Xiaodong Wang at the National Institute of Biological Sciences, Beijing.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9515(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab208910于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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IP |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
IP
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
靶标
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序列相似性
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain. -
结构域
The protein kinase domain is predicted to be catalytically inactive. - Information by UniProt
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数据库链接
- Entrez Gene: 74568 Mouse
- SwissProt: Q9D2Y4 Mouse
- Unigene: 207971 Mouse
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别名
- 9130019I15Rik antibody
- FLJ34389 antibody
- hMLKL antibody
see all
图片
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MLKL (phospho S345) was immunoprecipitated from 1mg of L-929 (Mouse connective tissue fibroblast cells) whole cell lysate treated with 20 ng/ml TNF alpha + 100 nM Smac mimetic + 20 µM z-VAD compound for 8h using ab196436 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab196436 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: L-929 whole cell lysate treated with 20 ng/ml TNF alpha+ 100 nM Smac mimetic+ 20 µM z-VAD compound for 8h;10 µg (Input).
Lane 2: ab196436 IP in L-929 whole cell lysate treated with 20 ng/ml TNF alpha+ 100 nM Smac mimetic+ 20 µM z-VAD compound for 8h.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196436 in L-929 whole cell lysate treated with 20 ng/ml TNF alpha+ 100 nM Smac mimetic+ 20 µM z-VAD compound for 8h.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196436).
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This Dot Blot data was generated using the same anti-MLKL (phospho S345) antibody clone, EPR9515(2), in a different buffer formulation (cat# ab196436).
Dot blot analysis of MLKL (phospho S345) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab196436 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (4)
ab208910 被引用在 4 文献中.
- Zhu Y et al. Angiotensin II triggers RIPK3-MLKL-mediated necroptosis by activating the Fas/FasL signaling pathway in renal tubular cells. PLoS One 15:e0228385 (2020). PubMed: 32134954
- Zhao P et al. Up-regulation of CHMP4B alleviates microglial necroptosis induced by traumatic brain injury. J Cell Mol Med 24:8466-8479 (2020). PubMed: 32585748
- Zhu Y et al. AT1 and AT2 receptors modulate renal tubular cell necroptosis in angiotensin II-infused renal injury mice. Sci Rep 9:19450 (2019). PubMed: 31857626
- Cui YL et al. Necroptosis as a potential therapeutic target in multiple organ dysfunction syndrome. Oncotarget 8:56980-56990 (2017). IHC-P ; Rat . PubMed: 28915647