概述

  • 产品名称
    Anti-MKP-1抗体
    参阅全部 MKP-1 一抗
  • 描述
    山羊多克隆抗体to MKP-1
  • 宿主
    Goat
  • 特异性
    The immunogen sequence for ab1351, anti MKP-1 antibody (aka DUSP1), shares 100% sequence homology to MKP-2 (aka DUSP4).
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human MKP-1 aa 355-367 (C terminal). The immunogen sequence for ab1351, anti MKP-1 antibody (aka DUSP1), shares 100% sequence homology to MKP-2 (aka DUSP4).
    Sequence:

    SYLQSPITTSPSC


    Database link: P28562

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

应用

Our Abpromise guarantee covers the use of ab1351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Most of the commercially available BSA and dry milk are contaminated with bovine IgG (even some of the higher purity grades of BSA); this might cause high background problems when labelled anti-goat secondary is used. The secondary might cross-react with bovine IgG since goat is closely related to cow.

靶标

  • 功能
    Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
  • 组织特异性
    Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
  • 序列相似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • CL 100 antibody
    • CL100 antibody
    • Dual Specificity Phosphatase 1 antibody
    • Dual specificity protein phosphatase 1 antibody
    • Dual specificity protein phosphatase hVH1 antibody
    • DUS1_HUMAN antibody
    • DUSP 1 antibody
    • Dusp1 antibody
    • HVH1 antibody
    • MAP kinase phosphatase 1 antibody
    • Mitogen-activated protein kinase phosphatase 1 antibody
    • MKP-1 antibody
    • MKP1 antibody
    • Protein tyrosine phosphatase CL100 antibody
    • Protein-tyrosine phosphatase CL100 antibody
    • PTPN10 antibody
    • Serine/threonine specific protein phosphatase antibody
    • VH1 antibody
    see all

图片

  • Western blot - Anti-MKP-1 antibody (ab1351)
    Western blot - Anti-MKP-1 antibody (ab1351)

    Endogenous MKP-1 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence Endogenous MKP-1 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKP-1 antibody (ab1351)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKP-1 antibody (ab1351)

    ab1351 (3.8 µg/ml) staining MKP-1 in paraffin-embedded Human prostate tissue. Steam antigen retrieval was performed with citrate buffer (pH 6).

文献

This product has been referenced in:
  • Shen J  et al. DUSP1 inhibits cell proliferation, metastasis and invasion and angiogenesis in gallbladder cancer. Oncotarget 8:12133-12144 (2017). Read more (PubMed: 28129656) »
  • Delehouzé C  et al. CDK/CK1 inhibitors roscovitine and CR8 downregulate amplified MYCN in neuroblastoma cells. Oncogene N/A:N/A (2013). Human . Read more (PubMed: 24317512) »

See all 4 Publications for this product

发表研究结果有使用 ab1351？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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11 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-MKP-1 antibody

 Below Average
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Chicken Cell lysate - whole cell (Thrombocyte)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
2 µg
Specification
Thrombocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Mrs. Katie Elliott

Verified customer

提交于 Sep 29 2016

Immunocytochemistry abreview for Anti-MKP-1 antibody

 Good
Abreviews
Application
Immunocytochemistry
Sample
Human Cultured Cells (Glioblastoma cells (U87MG, LN18,..) (all human))
Permeabilization
Yes - Triton x
Specification
Glioblastoma cells (U87MG, LN18,..) (all human)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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Mr. Matthias Dedobbeleer

Verified customer

提交于 May 04 2016

We are seeing no signal when using this product in IHC-P on mouse.

We recommend using a permeablization step with this product in IHC-P. Permeabilize with 0.25% Triton X-100 for 10 minutes at room temperature. Perform this step after antigen retrieval and before blocking.

Western blot abreview for Anti-MKP-1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (spinal cord homogenate)
Gel Running Conditions
Reduced Denaturing (10% SDS_PAGE)
Loading amount
20 µg
Treatment
sham or nerve transected
Specification
spinal cord homogenate
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Username

Abcam user community

Verified customer

提交于 Jun 17 2008

Western blot abreview for Anti-MKP-1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (HAPI cell line)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
400000 cells
Treatment
LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Specification
HAPI cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
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Abcam user community

Verified customer

提交于 Jun 13 2008

Western blot abreview for Anti-MKP-1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (BV2 cell line)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
400000 cells
Treatment
LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Specification
BV2 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Jun 10 2008

Western blot abreview for Anti-MKP-1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Microglia)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
400000 cells
Treatment
LPS 50 ng/mL or LPS 50 ng/mL +UO 10 uM
Specification
Primary Microglia
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
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Username

Abcam user community

Verified customer

提交于 Jun 10 2008

What concentration of protein was loaded onto the gel? What was the protocol used for this western blot? Ususally DUSP1 needs to be stimulated in order to see endogenus induction in cells. Thank you for your help.

Thank you for your enquiry. I apologize for the delay in answering your question due to the holidays. The protocol used to test this antibody is below. 35ug protein per lane was loaded and its concentration would have been 2.5mg/ml in SDS Page loa...

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Does this antibody detect endogenous DUSP1? I can use it to detect recombinant protein, but am not seeing a band in cell lysates. I am using 100ug cell lysate, and using the antibody at 2ug/ml.

Thank you for your enquiry. The Western on the datasheet is detecting endogenous DUSP1 in Hela. In order to further troubleshoot why this antibody is not giving you any signal, it would be helpful if you could fill out the form at the link I am i...

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I am performing WB using a thyroid cancer cell line and a prostate cancer cell line (LNCAP). I am obtaining a band at the predicted weight of 40kDa, but also another band at around 45kDa. I am wondering if this extra band is DUSP4? Has this antibody sh...

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Thank you for your enquiry and for your patience. The originator of this antibody has informed me that they only observed a single band in HeLa lysate and we have not had any other feedback yet regarding the use of ab1351. However, looking at the seque...

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