重组Anti-Mitofusin 2 + Mitofusin 1抗体[EPR19792] (ab205235)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19792] to Mitofusin 2 + Mitofusin 1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
概述
-
产品名称
Anti-Mitofusin 2 + Mitofusin 1抗体[EPR19792]
参阅全部 Mitofusin 2+Mitofusin 1 一抗 -
描述
兔单克隆抗体[EPR19792] to Mitofusin 2 + Mitofusin 1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Human Mitofusin 2 and Mitofusin 1 recombinant protein fragments; Human fetal kidney and fetal liver lysates; HeLa whole cell lysate. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19792 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab205235于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/50.
|
|
WB |
1/2000. Detects a band of approximately 80 kDa (predicted molecular weight: 84, 86 kDa).
|
|
ICC/IF |
1/500.
|
|
IP |
1/40.
|
说明 |
---|
Flow Cyt (Intra)
1/50. |
WB
1/2000. Detects a band of approximately 80 kDa (predicted molecular weight: 84, 86 kDa). |
ICC/IF
1/500. |
IP
1/40. |
靶标
-
细胞定位
Mitofusin 2: Mitochondrion outer membrane. Colocalizes with BAX during apoptosis. Mitofusin 1: Cytoplasm and Mitochondrion outer membrane. -
数据库链接
- Entrez Gene: 55669 Human
- Entrez Gene: 9927 Human
- Omim: 608506 Human
- Omim: 608507 Human
- SwissProt: O95140 Human
- SwissProt: Q8IWA4 Human
- Unigene: 376681 Human
- Unigene: 478383 Human
图片
-
All lanes : Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] (ab205235) at 1/2000 dilution
Lane 1 : Human Mitofusin 2 recombinant protein fragment
Lane 2 : Human Mitofusin 1 recombinant protein fragment
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 84, 86 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Human Mitofusin 2 recombinant protein fragment contains aa151-506 with a His-Tag®. Human Mitofusin 1 recombinant protein fragment contains aa130-485 with a His-Tag®.
-
All lanes : Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] (ab205235) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 84, 86 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 15 seconds; Lane 3: 3 seconds.
The expression profile is consistent with the literature (PMID 14561718; PMID 25574749).
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mitofusin 2 + Mitofusin 1 with ab205235 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mitofusin 2 + Mitofusin 1 with ab205235 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mitochondrial staining on HeLa cells.
The nuclear counter stain is DAPI (blue). Cox IV is detected with ab33985 (anti-Cox IV mouse mAb) at 1/1000 dilution followed by ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab205235 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab33985 at 1/1000 dilution followed by ab150077 at 1/1000 dilution. -
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mitofusin 2 + Mitofusin 1 with ab205235 at 1/50 dilution (red) compared with aRabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.
-
Mitofusin 2 + Mitofusin 1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab205235 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab205235 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10 μg (Input).
Lane 2: ab205235 IP in HeLa whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab205235 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab205235 尚未被引用在任何文献中。