重组Anti-PDK1抗体[SP270] - C-terminal (ab227682)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP270] to PDK1 - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
概述
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产品名称
Anti-PDK1抗体[SP270] - C-terminal
参阅全部 PDK1 一抗 -
描述
兔单克隆抗体[SP270] to PDK1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human kidney, liver, stomach, heart, pancreas, testis and liver hepatocellular carcinoma tissues.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by Protein A/G. -
克隆
单克隆 -
克隆编号
SP270 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227682于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100.
Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature. |
说明 |
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IHC-P
1/100. Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature. |
靶标
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功能
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
组织特异性
Expressed predominantly in the heart. -
序列相似性
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 5163 Human
- Omim: 602524 Human
- SwissProt: Q15118 Human
- Unigene: 470633 Human
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别名
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
- HGNC:8809 antibody
- Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
see all
图片
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Formalin-fixed, paraffin-embedded human kidney tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human heart tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human testis tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab227682 被引用在 1 文献中.
- Sun J et al. Circ_0000284 facilitates the growth, metastasis and glycolysis of intrahepatic cholangiocarcinoma through miR-152-3p-mediated PDK1 expression. Histol Histopathol N/A:18544 (2022). PubMed: 36331285