Anti-Milk Fat Globule 1抗体[SPM291] (ab17787)
Key features and details
- Mouse monoclonal [SPM291] to Milk Fat Globule 1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Milk Fat Globule 1抗体[SPM291]
参阅全部 Milk Fat Globule 1 一抗 -
描述
小鼠单克隆抗体[SPM291] to Milk Fat Globule 1 -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
The details of the immunogen for this antibody are not available.
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阳性对照
- IHC: Human breast carcinoma, renal cell carcinoma, pancreas and lung carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.0268% PBS, BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
SPM291 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17787于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
Medin is the main constituent of aortic medial amyloid. -
组织特异性
Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas. -
序列相似性
Contains 1 EGF-like domain.
Contains 2 F5/8 type C domains. -
翻译后修饰
Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273. -
细胞定位
Membrane. Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4240 Human
- Omim: 602281 Human
- SwissProt: Q08431 Human
- Unigene: 3745 Human
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别名
- BA46 antibody
- Breast epithelial antigen BA46 antibody
- EDIL1 antibody
see all
图片
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IHC image of ab17787 staining in human carinoma Renal Cell formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
IHC image of ab17787 staining in human carinoma breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Milk Fat Globule 1 with ab18878 at 1 ug/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling Milk Fat Globule 1 with ab18878 at 1 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab17787 被引用在 6 文献中.
- Xiao L et al. Regulation of striatal cells and goal-directed behavior by cerebellar outputs. Nat Commun 9:3133 (2018). PubMed: 30087345
- Baumgartner M et al. Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self-amplifying radial glial cells. Development 145:N/A (2018). PubMed: 30093551
- Yu L et al. MFGE8 regulates TGF-ß-induced epithelial mesenchymal transition in endometrial epithelial cells in vitro. Reproduction 152:225-33 (2016). ICC/IF, WB ; Human . PubMed: 27340235
- Soki FN et al. Polarization of Prostate Cancer-associated Macrophages Is Induced by Milk Fat Globule-EGF Factor 8 (MFG-E8)-mediated Efferocytosis. J Biol Chem 289:24560-72 (2014). IHC-P ; Human . PubMed: 25006249
- Yu L et al. Tumor necrosis factor a up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor ?B activation, resulting in cell migration of epithelial cells. Fertil Steril N/A:N/A (2013). Human . PubMed: 24262600
- Franchi A et al. Expression of milk fat globule EGF-factor 8 (MFG-E8) mRNA and protein in the human endometrium and its regulation by prolactin. Mol Hum Reprod 17:360-71 (2011). WB, ICC/IF, IHC-P, IHC-Fr ; Human . PubMed: 21177637