Key features and details
- Mouse monoclonal [OX18] to MHC class I
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
产品名称Anti-MHC class I抗体[OX18]
参阅全部 MHC class I 一抗
描述小鼠单克隆抗体[OX18] to MHC class I
经测试应用适用于: IHC-Fr, Flow Cytmore details
Other Immunogen Type corresponding to MHC class I. Rat spleen membrane glycoproteins
- IHC-Fr: Normal rat placenta tissue. Flow Cyt: Lewis rat splenocytes.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
纯度Protein G purified
Our Abpromise guarantee covers the use of ab6405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.|
相关性MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
细胞定位Cell Membrane; Type I membrane protein.
- A 28 antibody
- A 9 antibody
- Antigen presenting molecule antibody
ab170190) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6405) or mouse IgG1κ (ab170190) isotype (1x 10ab150117) was used at 1/2000 dilution for 30 min on ice.
IHC image of MHC Class I staining in a section of frozen normal rat placenta*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab6405 (shown in green) at 1 μg/ml. The section was then incubated with ab150117 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488, 1/1000)) presabsorbed for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount® .
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
*Tissue obtained from Charles River.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.