参阅全部 MGAT5 一抗
经测试应用适用于: WBmore details
种属反应性与反应: Mouse, Human
预测可用于: Rat, Sheep, Horse, Dog
- This antibody gave a positive signal in the following Whole Cell Lysates: TE 671, Jurkat, Jurkat - Staurosporine Treated, MEF1 (data not shown) and HeLa - Hydroxyurea Treated (48hr, 2uM) (data not shown).
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab68595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 84 kDa).Can be blocked with Human MGAT5 peptide (ab68594).|
功能Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
通路Protein modification; protein glycosylation.
序列相似性Belongs to the glycosyltransferase 18 family.
细胞定位Golgi apparatus membrane.
- Information by UniProt
- 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A antibody
- 6-N-acetylglucosaminyltransferase antibody
- Alpha 1,3(6) mannosylglycoprotein antibody
All lanes : Anti-MGAT5 antibody (ab68595) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
MGAT5 has a predicted molecular weight of 84 kDa; however it is a novel protein that is predicted to be extensively glycosylated (SwissProt data). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab68595 has not yet been referenced specifically in any publications.