This antibody gave a positive signal in the following Whole Cell Lysates:
TE 671, Jurkat, Jurkat - Staurosporine Treated, MEF1 (data not shown) and HeLa - Hydroxyurea Treated (48hr, 2uM) (data not shown).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 84 kDa).Can be blocked with Human MGAT5 peptide (ab68594).
Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
MGAT5 has a predicted molecular weight of 84 kDa; however it is a novel protein that is predicted to be extensively glycosylated (SwissProt data).
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.