Anti-Metallothionein抗体(ab192385)
Key features and details
- Rabbit polyclonal to Metallothionein
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Metallothionein抗体
参阅全部 Metallothionein 一抗 -
描述
兔多克隆抗体to Metallothionein -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Dog, Pig, Cynomolgus monkey, Orangutan -
免疫原
Recombinant full length protein corresponding to Human Metallothionein aa 1 to the C-terminus.
Database link: P02795 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab192385 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab192385于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 6 kDa.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 6 kDa. |
靶标
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功能
Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. -
序列相似性
Belongs to the metallothionein superfamily. Type 1 family. -
结构域
Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines. - Information by UniProt
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数据库链接
- Entrez Gene: 403800 Dog
- Entrez Gene: 4489 Human
- Entrez Gene: 4502 Human
- Entrez Gene: 17750 Mouse
- Entrez Gene: 397417 Pig
- Omim: 156350 Human
- Omim: 156360 Human
- SwissProt: P68303 Cynomolgus monkey
see all -
别名
- CES 1 antibody
- CES1 antibody
- Metallothionein 1A antibody
see all
图片
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ab192385 staining Metallothionein in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with ab64226 for 10 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/150) for 30 minutes. An undiluted HRP-conjugated Goat polyclonal (1/250) was used as the secondary antibody
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Western blot analysis of extracts of HeLa cells useing ab192385.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab192385 被引用在 6 文献中.
- Zeng Z et al. Reducing Endogenous Labile Zn May Help to Reduce Smooth Muscle Cell Injury around Vascular Stents. Int J Mol Sci 23:N/A (2022). PubMed: 35563532
- Liu X et al. Metallothionein 2A (MT2A) controls cell proliferation and liver metastasis by controlling the MST1/LATS2/YAP1 signaling pathway in colorectal cancer. Cancer Cell Int 22:205 (2022). PubMed: 35642057
- Schwarz M et al. Copper interferes with selenoprotein synthesis and activity. Redox Biol 37:101746 (2020). PubMed: 33059313
- Schwarz M et al. Crosstalk of Nrf2 with the Trace Elements Selenium, Iron, Zinc, and Copper. Nutrients 11:N/A (2019). PubMed: 31491970
- Guo JZ et al. The role of metallothionein in a dinitrofluorobenzene-induced atopic dermatitis-like murine model. Sci Rep 8:11129 (2018). PubMed: 30042397
- Chen J et al. From the Cover: Zinc Deficiency Worsens and Supplementation Prevents High-Fat Diet Induced Vascular Inflammation, Oxidative Stress, and Pathological Remodeling. Toxicol Sci 153:124-36 (2016). PubMed: 27370414