概述

  • 产品名称
    Anti-Metabotropic Glutamate Receptor 5抗体
    参阅全部 Metabotropic Glutamate Receptor 5 一抗
  • 描述
    兔多克隆抗体to Metabotropic Glutamate Receptor 5
  • 宿主
    Rabbit
  • 特异性
    ab53090 detects endogenous levels of total Metabotropic Glutamate Receptor 5 protein.
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
  • 免疫原

    N-terminal synthetic peptide derived from human Metabotropic Glutamate Receptor 5.

  • 阳性对照
    • WB: Mouse brain extracts IHC-P: Human brain tissue

性能

应用

Our Abpromise guarantee covers the use of ab53090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 22962568
ICC/IF 1/100. PubMed: 19364772
IHC-P 1/50 - 1/100.
ELISA 1/5000.
WB 1/300 - 1/1000. Predicted molecular weight: 132 kDa.

靶标

  • 功能
    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
  • 序列相似性
    Belongs to the G-protein coupled receptor 3 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Glutamate receptor metabotropic 5 antibody
    • GPRC1E antibody
    • Grm5 antibody
    • GRM5_HUMAN antibody
    • Metabotropic glutamate receptor 5 antibody
    • Metabotropic glutamate receptor 5 variant F antibody
    • Metabotropic glutamate receptor 5 variant G antibody
    • Metabotropic glutamate receptor 5 variant H antibody
    • mGlu5 antibody
    • mGluR5 antibody
    • PPP1R86 antibody
    • Protein phosphatase 1 regulatory subunit 86 antibody
    see all

图片

  • All lanes : Anti-Metabotropic Glutamate Receptor 5 antibody (ab53090) at 1/300 dilution

    Lane 1 : Mouse brain extracts
    Lane 2 : Mouse brain extracts with blocking peptide

    Predicted band size: 132 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

  • ab53090 staining Metabotropic Glutamate Receptor 5 (red) in rat hippocampla neuron by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 24 hours at 22°C. An Alexa Fluor® 594-conjugated chicken anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Cyan - pre-synaptic marker Bassoon.

    See Abreview

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab53090 at 1/50 dilution, in the presence (right) and absence (left) of blocking peptide.
  • ab53090 staining Metabotropic Glutamate Receptor 5 in rat brain tissue sections by Immunohistochemistry (Free Floating).
    Animals were perfused fixed with 4% paraformaldehyde with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using ab53090 at a 1/300 dilution. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit used at a 1/1000 dilution.

    See Abreview

文献

This product has been referenced in:
  • Dai J  et al. Improved retinal function in RCS rats after suppressing the over-activation of mGluR5. Sci Rep 7:3546 (2017). Read more (PubMed: 28615682) »
  • Goniotaki D  et al. Inhibition of group-I metabotropic glutamate receptors protects against prion toxicity. PLoS Pathog 13:e1006733 (2017). Read more (PubMed: 29176838) »
See all 10 Publications for this product

客户评价及客户问答

1-7 of 7 Q&A

Answer

Thank you for your reply.

The blocking peptide for the Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) is now available to purchase under the catalogue number ab139974. The details of this product can be viewed from the following link:

https://www.abcam.com/Metabotropic-Glutamate-Receptor-5-peptide-ab139974.html

If you have any further questions, please do not hesitate to ask.

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Answer

Sorry for the delay in getting back to you.

I have had confirmation that we do have the blocking peptide available for the rabbit monoclonal anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316). If you would like for me to add this to the catalogue for your customer please do let me know.

I have now arranged for the replacement antibody ab76316 to be sent to you. This is on the order number1111586. Please bear in mind that the item may be moved from this order before shipping through consolidation of the orders. If you would like to track the item please quote "FOCR CCE4186704" to any of our customer service representatives.

I hope this replacement works well for the customer. If they continue to have any problems or if I can be of any further assistance, please do letme know.

Read More

Answer

Thank you for contacting us and reporting the problems you have been experiencing with the Anti-Metabotropic Glutamate Receptor 5 antibody (ab53090).

I am sorry to hear that this antibody has not been providing the expected results. Having reviewed the protocol used with the antibody I would have expected the antibody to work. It may unfortunately be that you have received a faulty vial. I am sorry for this and the inconvenience caused.

I would be happy to provide a replacement antibody or a credit note. Unfortunately, we do not currently have an alternative lot of ab53090 in stock. We do however have an alternative antibody, ab76316, which may be suitable. Please let me know if you would like me to send this as a replacement.

I look forward to hearing how you would like to proceed.

Read More

Answer

Thank you for your reply.

I have processed your request to receive ab17722 as an free of charge replacement for ab53090.

Please let me know if there is anything else I can help you with.

Read More

Answer

Thank you for your reply.

As the customer is still not happy with the performance of ab53090, I am happy to replace or refund the antibody.

If you could provide me with original Abcam order information, and the abID of the replacement antibody requested, if that is the case, I will be happy process the refund or replacement.

I look forward to your reply and I am sorry for all the issues the customer has been having with this antibody.

Read More

Question

Below is supplied the information about the immunocytochemistry procedure you have requested. I look forward to hearing your thoughts on the nuclear staining problem.

1) Abcam product code ab53090

2) Abcam order reference number or product batch number: ######

3) Description of the problem: Nuclear staining, should be membranous

4) Sample preparation:

SpeciesMouse

Type of sample: Paraformaldehyde fixed primary cell culture

Sample preparation

Positive control Presence of flourescens in sample

Negative control Secondary antibody alone to subtract background staining

5) Fixation step

Yes/No

If yes: Fixative agent and concentration Paraformaldehyde 4%

Fixation time 10 min

Fixation temperature 4 degrees C

6) Antigen retrieval method None

7) Permeabilization method:

Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers? 0.1 % Triton-X for 30 min. Not added to any dilution buffers.

Permeabilizing agent and concentration: 0.1 % Triton-X in PBS

8) Blocking agent (eg BSA, serum…): BSA

Concentration 1 %

Blocking time 1.5 hours

Blocking temperature Room temp

9) Endogenous peroxidases blocked? No

Endogenous biotins blocked? No

10) Primary antibody (If more than one was used, describe in “additional notes”) : mGluR5 (ab53090)

Concentration or dilution 1:50, 1:100 and 1:200

Diluent buffer PBS with 1 % BSA

Incubation time Overnight at 4 degrees C

11) Secondary antibody:

Species:Goat

Reacts against:Rabbit

Concentration or dilution 1:1000

Diluent buffer PBS with 1 % BSA

Incubation time 2 hours

Fluorochrome or enzyme conjugate Alexa Flour 488

12) Washing after primary and secondary antibodies:

Buffer PBS

Number of washes 5 x 5 min

13) Detection method Confocal microscopy

14) How many times have you run this staining? Once

Do you obtain the same results every time? NA

What steps have you altered to try and optimize the use of this antibody? NA

Ilook forward to receiving your reply.

Thank you for your inquiry.

Read More
Answer

Thank you for providing that additional information.


Would you be able to try to optimize without the permeabilization step? Since the immunogen is N-terminal, this antibody should bind to the extracellular portion of mGluR5. Just omit the Triton X step from the protocol.


Plus, do you have normal goat serum? You can try changing the blocking buffer to 10% goat serum for 1 hr at RT in case the block had anything to do with non-specific binding.


Also try incubating the secondary for 1 hr at RT instead of 2 hrs in case this led to some non-specificity.


I hope this information helps. If you try the protocol suggestions and they do not improve your results, please let me know and we'd be happy to replace or refund the product for you.

Read More

Answer

I am sorry about the problems that you are having with ab53090 in IHC-P. I have looked over the protocol information that you sent and compared it to the Abreview information that we have and the only recommendation that I can make it to increase the primary antibody concentration to 1/50 dilution, with the citrate buffer antigen retrieval method, as the rest of the protocol that you are using should work. If increasing the antibody concentration does not prove to be successful then I would be happy to send you a replacement antibody (either a new vial of the same antibody or a different Metabotropic Glutamate Receptor 5 antibody) or process a refund. I look forward to your response and helping you resolve this issue.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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