Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235)抗体(ab5662)
Key features and details
- Rabbit polyclonal to Met (c-Met) (phospho Y1230 + Y1234 + Y1235)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235)抗体
参阅全部 Met (c-Met) 一抗 -
描述
兔多克隆抗体to Met (c-Met) (phospho Y1230 + Y1234 + Y1235) -
宿主
Rabbit -
特异性
The phosphospecific antibody that has been generated does not distinguish between the dually (pYpY 1234/1235) and triply (pYpYpY1230/1234/1235) phosphorylated forms of c-Met, both of which are likely to represent activated forms of this receptor. -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic phosphopeptide derived from the region of human c-Met that contains tyrosines 1230, 1234 and 1235. (Peptide available as ab41697.)
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阳性对照
- WB: HEK-293T whole cell lysate.
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常规说明
Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyr-xx- x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1) receptor and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of tyrosines 1234 and 1235 has been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, Erk1&2, JNK, PLC-ã, and STAT3).
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Met protein. The final product is generated by affinity chromatography using a c Met-derived peptide that is phosphorylated at tyrosines 1230, 1234, 1235. -
Primary antibody说明
Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyr-xx- x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1) receptor and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of tyrosines 1234 and 1235 has been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, Erk1&2, JNK, PLC-ã, and STAT3). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab5662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 169 kDa. | |
| ELISA | Use at an assay dependent concentration. |
靶标
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功能
Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival. -
疾病相关
Note=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
Note=Defects in MET may be associated with gastric cancer.
Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain. -
结构域
The kinase domain is involved in SPSB1 binding. -
翻译后修饰
Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4233 Human
- Entrez Gene: 17295 Mouse
- Entrez Gene: 24553 Rat
- Omim: 164860 Human
- SwissProt: P08581 Human
- SwissProt: P16056 Mouse
- SwissProt: P97523 Rat
- Unigene: 132966 Human
see all -
别名
- AUTS9 antibody
- c met antibody
- D249 antibody
see all
图片
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Western blot - Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235) antibody (ab5662)All lanes : Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235) antibody (ab5662) at 1/1000 dilution
Lane 1 : Unstimulated (-), HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lanes 2-5 : Stimulated (+) with HGF, HEK-293T whole cell extract
Secondary
All lanes : Goat F(ab’)2 antirabbit IgG HRP conjugatePeptide Competition:
Prior primary antibody incubation:
1 and 2 - no peptide,
3 - non-phosphopeptide corresponding to the immunogen,
4 -generic phosphotyrosine-containing peptide),
5 - phosphopeptide immunogen.
SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C.
Bands were detected using the Pierce SuperSignal method.
The data show that only the phosphopeptide corresponding to c Met pYpYpY1230/1234/1235] block the antibody signal, demonstrating the specificity of the antibody.
Note: There are three isoforms of c Met, two of which are recognized by this antibody.
实验方案
文献 (22)
ab5662 被引用在 22 文献中.
- Ueki R et al. A chemically unmodified agonistic DNA with growth factor functionality for in vivo therapeutic application. Sci Adv 6:eaay2801 (2020). PubMed: 32270033
- Imafuku A et al. Rat Mesenchymal Stromal Cell Sheets Suppress Renal Fibrosis via Microvascular Protection. Stem Cells Transl Med 8:1330-1341 (2019). PubMed: 31675167
- Bonan NF et al. Inhibition of HGF/MET signaling decreases overall tumor burden and blocks malignant conversion in Tpl2-related skin cancer. Oncogenesis 8:1 (2019). PubMed: 30631034
- Zhang J et al. MicroRNA-206 exerts anti-oncogenic functions in esophageal squamous cell carcinoma by suppressing the c-Met/AKT/mTOR pathway. Mol Med Rep 19:1491-1500 (2019). PubMed: 30569129
- Jin Q et al. Hypoxia-inducible factor-1 signaling pathway influences the sensitivity of HCC827 cells to gefitinib. Oncol Lett 17:4034-4043 (2019). PubMed: 30881515
