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Tags & Cell Markers Cell Type Markers Tumor Associated

Anti-Mesothelioma抗体[HBME-1] (ab2383)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelioma antibody [HBME-1] (ab2383)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelioma antibody [HBME-1] (ab2383)

Key features and details

  • Mouse monoclonal [HBME-1] to Mesothelioma
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgM

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概述

  • 产品名称

    Anti-Mesothelioma抗体[HBME-1]
    参阅全部 Mesothelioma 一抗
  • 描述

    小鼠单克隆抗体[HBME-1] to Mesothelioma
  • 宿主

    Mouse
  • 特异性

    This antibody reacts with an unknown antigen on microvilli of mesothelioma cells. It stains normal mesothelial cells as well as epithelial mesothelimias in a thick membrane pattern due to abundant lung microvilli on the surface of these cells.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • 免疫原

    Suspension of human mesothelioma cells from patients with malignant epithelial mesothelioma.

  • 阳性对照

    • Mesothelioma.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • 纯度

    Purified IgM
  • 克隆

    单克隆
  • 克隆编号

    HBME-1
  • 骨髓瘤

    unknown
  • 同种型

    IgM
  • 轻链类型

    unknown
  • 研究领域

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated

相关产品

  • Alternative Versions

    • Anti-Mesothelioma antibody [HBME-1], prediluted (ab75307)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
应用 Ab评论 说明
IHC-P
说明
  • 应用说明
    IHC: This antibody may be diluted to a titer of 1:12-1:25 in an ABC method.
    We suggest an incubation period of 60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
    Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
  • 靶标

    • 相关性

      Mesothelioma is a rare form of carcinoma of the mesothelial cells which that normally line the body cavities, including the pleura, peritoneum, pericardium, and testis. It may be localized or diffuse. Most, but not all, cases of pleural malignant mesothelioma are associated with asbestos exposure.
    • 别名

      • Malignant mesothelioma susceptibility to antibody
      • MMS antibody

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelioma antibody [HBME-1] (ab2383)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelioma antibody [HBME-1] (ab2383)
      ab2383 staining human mesothelioma by IHC-P.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelioma antibody [HBME-1] (ab2383)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelioma antibody [HBME-1] (ab2383)
      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human mesothelioma tissue, staining Mesothelioma with ab2383.

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab2383?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2383 被引用在 1 文献中.

    • Chernova T  et al. Long-Fiber Carbon Nanotubes Replicate Asbestos-Induced Mesothelioma with Disruption of the Tumor Suppressor Gene Cdkn2a (Ink4a/Arf). Curr Biol 27:3302-3314.e6 (2017). PubMed: 29112861

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Question

    What is the concentration of this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 16 2012

    Answer

    Thank you for contacting Abcam regarding ab2383.


    The average concentration of this antibody is 45mg/L. If you have a specific lot number, I can check for that concentration as well.


    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Abcam Scientific Support

    回复于 Apr 16 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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