概述

  • 产品名称
    Anti-MERTK抗体[Y323]
    参阅全部 MERTK 一抗
  • 描述
    兔单克隆抗体[Y323] to MERTK
  • 宿主
    Rabbit
  • 经测试应用
    适用于: Blocking, WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human MERTK aa 1-100 (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: HEK-293 and K562 cell lysate. IHC-P: Human tonsil and lymphoma tissues. IP: MERTK IP in HEK-293 cell lysate.
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab52968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Blocking Use at an assay dependent concentration. PubMed: 19204785
WB 1/2000. Detects a band of approximately 180 kDa.

For unpurified use at 1/500.

IP 1/20.

For unpurified use at 1/50.

IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • 应用说明
    Is unsuitable for Flow Cyt or ICC/IF.
  • 靶标

    • 功能
      In case of filovirus infection, seems to function as a cell entry factor.
    • 组织特异性
      Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.
    • 疾病相关
      Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    • 序列相似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • c MER antibody
      • c mer proto oncogene tyrosine kinase antibody
      • c-mer antibody
      • cMER antibody
      • cmer protooncogene tyrosine kinase antibody
      • Eyk antibody
      • MER antibody
      • MER receptor tyrosine kinase antibody
      • MERK antibody
      • MERPEN antibody
      • Mertk antibody
      • MERTK c-mer proto-oncogene tyrosine kinase antibody
      • MERTK_HUMAN antibody
      • MGC133349 antibody
      • nmf12 antibody
      • Nyk antibody
      • Proto oncogene tyrosine protein kinase MER antibody
      • Proto oncogene tyrosine protein kinase MER precursor antibody
      • Proto-oncogene c-Mer antibody
      • Receptor tyrosine kinase MerTK antibody
      • RP38 antibody
      • STK kinase antibody
      • Tyrosine-protein kinase Mer antibody
      see all

    图片

    • ab52968 used at a 1/200 dilution, 18 hours at 4°C, 5% BSA in Tween PBS.

      Positive cell line: A172

      Negative cell lines: CV1 and HeLa.

      Secondary used is a goat anti-rabbit polyclonal-HRP at 1/10000 dilution.

      Blocked with 5% milk for 1 hour at RT.

      See Abreview

    • All lanes : Anti-MERTK antibody [Y323] (ab52968) at 1/2000 dilution (purified)

      Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
      Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Observed band size: 140-180 kDa
      why is the actual band size different from the predicted?



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Lane 1: ab52968 (purified) at 1/20 immunoprecipitating MERTK in HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate.

      Lane 2 - PBS.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MERTK with purified ab52968 at 1/500.

      Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

      Negative control using PBS instead of primary antibody (Inset).

    • Anti-MERTK antibody [Y323] (ab52968) at 1/1000 dilution (unpurified) + HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Observed band size: 180 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labeling MERTK with unpurified ab52968 at a 1/50 dilution.

    文献

    This product has been referenced in:
    • Waterborg CEJ  et al. Protective Role of the MER Tyrosine Kinase via Efferocytosis in Rheumatoid Arthritis Models. Front Immunol 9:742 (2018). Read more (PubMed: 29706963) »
    • Kwon JY  et al. Relevance of placental type I interferon beta regulation for pregnancy success. Cell Mol Immunol N/A:N/A (2018). Read more (PubMed: 29907882) »
    See all 29 Publications for this product

    客户评价及客户问答

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    1-2 of 2 Abreviews

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    提交于 Nov 14 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (cell lines)
    Loading amount
    20 µg
    Specification
    cell lines
    Gel Running Conditions
    Reduced Denaturing (4-15% Tris-glycine gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Mr. Nir Drayman

    Verified customer

    提交于 Mar 05 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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