Name: Anti-MERTK antibody [Y323]
Brand: RabMAb
SKU: ab52968
Rating: 5 (4 reviews)

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Western blot - Anti-MERTK antibody [Y323] (ab52968)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y323] to MERTK
Suitable for: WB, IP, IHC-P, IHC-Fr
Knockout validated
Reacts with: Human

Carrier Free HRP

产品名称

Anti-MERTK抗体[Y323]
参阅全部 MERTK 一抗
描述

兔单克隆抗体[Y323] to MERTK
宿主

Rabbit
经测试应用

适用于: WB, IP, IHC-P, IHC-Frmore details
不适用于: Flow Cyt or ICC/IF
种属反应性

与反应: Human
免疫原

Synthetic peptide within Human MERTK aa 1-100 (N terminal). The exact sequence is proprietary.
阳性对照
- WB: HEK-293 and K562 cell lysate. IHC-P: Human tonsil and lymphoma tissues. IP: MERTK IP in HEK-293 cell lysate. IHC-Fr: Hu Spleen tissue sections.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

Y323
同种型

IgG
研究领域

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HEK-293 whole cell lysate (ab7902)
- K-562 whole cell lysate (ab7911)
Recombinant Protein
- Recombinant human MERTK (mutated A708S) protein (ab167957)

The Abpromise guarantee

Abpromise™承诺保证使用ab52968于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(1)	1/2000. Detects a band of approximately 180 kDa. For unpurified use at 1/500.
IP		1/20. For unpurified use at 1/50.
IHC-P	(3)	1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
IHC-Fr		Use at an assay dependent concentration.

说明
WB 1/2000. Detects a band of approximately 180 kDa. For unpurified use at 1/500.
IP 1/20. For unpurified use at 1/50.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
IHC-Fr Use at an assay dependent concentration.

应用说明

Is unsuitable for Flow Cyt or ICC/IF.

功能

In case of filovirus infection, seems to function as a cell entry factor.
组织特异性

Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.
疾病相关

Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
序列相似性

Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
细胞定位

Membrane.
Target information above from: UniProt accession Q12866 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 10461 Human
- Omim: 604705 Human
- SwissProt: Q12866 Human
- Unigene: 306178 Human
别名
- c MER antibody
- c mer proto oncogene tyrosine kinase antibody
- c-mer antibody
- cMER antibody
- cmer protooncogene tyrosine kinase antibody
- Eyk antibody
- MER antibody
- MER receptor tyrosine kinase antibody
- MERK antibody
- MERPEN antibody
- Mertk antibody
- MERTK c-mer proto-oncogene tyrosine kinase antibody
- MERTK_HUMAN antibody
- MGC133349 antibody
- nmf12 antibody
- Nyk antibody
- Proto oncogene tyrosine protein kinase MER antibody
- Proto oncogene tyrosine protein kinase MER precursor antibody
- Proto-oncogene c-Mer antibody
- Receptor tyrosine kinase MerTK antibody
- RP38 antibody
- STK kinase antibody
- Tyrosine-protein kinase Mer antibody
see all

Western blot - Anti-MERTK antibody [Y323] (ab52968)

All lanes : Anti-MERTK antibody [Y323] (ab52968) at 1/500 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MERTK knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Lanes 1 - 2: Merged signal (red and green). Green - ab52968 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab52968 was shown to specifically react with MERTK in wild-type HAP1 cells as signal was lost in MERTK knockout cells. Wild-type and MERTK knockout samples were subjected to SDS-PAGE. The membrane was blocked with 0. Ab52968 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-MERTK antibody [Y323] (ab52968)

ab52968 used at a 1/200 dilution, 18 hours at 4°C, 5% BSA in Tween PBS.

Positive cell line: A172

Negative cell lines: CV1 and HeLa.

Secondary used is a goat anti-rabbit polyclonal-HRP at 1/10000 dilution.

Blocked with 5% milk for 1 hour at RT.
See Abreview
Western blot - Anti-MERTK antibody [Y323] (ab52968)

All lanes : Anti-MERTK antibody [Y323] (ab52968) at 1/2000 dilution (purified)

Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

Observed band size: 140-180 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation - Anti-MERTK antibody [Y323] (ab52968)

Lane 1: ab52968 (purified) at 1/20 immunoprecipitating MERTK in HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate.

Lane 2 - PBS.

For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MERTK with purified ab52968 at 1/500.

Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

Negative control using PBS instead of primary antibody (Inset).
Immunohistochemistry (Frozen sections) - Anti-MERTK antibody [Y323] (ab52968)

IHC image of MERTK staining in a section of frozen normal human spleen performed on a Leica BOND^TM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab52968, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-MERTK antibody [Y323] (ab52968)

Anti-MERTK antibody [Y323] (ab52968) at 1/1000 dilution (unpurified) + HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg

Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Observed band size: 180 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labeling MERTK with unpurified ab52968 at a 1/50 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-MERTK antibody [Y323] (ab52968)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab52968？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab52968 被引用在 51 文献中.

Gillen KM et al. QSM is an imaging biomarker for chronic glial activation in multiple sclerosis lesions. Ann Clin Transl Neurol 8:877-886 (2021). PubMed: 33704933
Tang C et al. A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface. Commun Biol 4:161 (2021). PubMed: 33547385
Giroud P et al. Expression of TAM-R in Human Immune Cells and Unique Regulatory Function of MerTK in IL-10 Production by Tolerogenic DC. Front Immunol 11:564133 (2020). PubMed: 33101282
Park TH et al. Crystal Structure of the Kinase Domain of MerTK in Complex with AZD7762 Provides Clues for Structure-Based Drug Development. Int J Mol Sci 21:N/A (2020). PubMed: 33114206
Vullings J et al. Selective Increment of Synovial Soluble TYRO3 Correlates with Disease Severity and Joint Inflammation in Patients with Rheumatoid Arthritis. J Immunol Res 2020:9690832 (2020). PubMed: 32964059

View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-10 of 10 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Ciliary Body)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH 6 & TRIS pH 9

Permeabilization

Yes - 0.3% Triton X-100

Specification

Ciliary Body

Blocking step

Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Fixative

Formaldehyde

Dr. Dylan Windell

Verified customer

提交于 May 13 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Blood vessel-Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH9

Permeabilization

Specification

Blood vessel-Brain

Blocking step

(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

提交于 Nov 30 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Lung)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate

Permeabilization

Specification

Lung

Blocking step

PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

提交于 Nov 14 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (cell lines)

Loading amount

20 µg

Specification

cell lines

Gel Running Conditions

Reduced Denaturing (4-15% Tris-glycine gel)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mr. Nir Drayman

Verified customer

提交于 Mar 05 2012

Question

Our customer is looking for the isotype control for ab52968 for FACS, ICC/IF and some inhibitory assay. As it was indicated in the reference article of ab52968, also the primary ab ab52968 and its isotype control were purchased from Abcam:

Carr AJ et al. Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay. Mol Vis 15:283-95 (2009). WB.

p.285 To examine the role of MERTK in phagocytosis, we incubated HESC-RPE cells with rabbit monoclonal antibody to 1:40 MERTK or 1:40 control rabbit IgG (both Abcam, Cambridge, UK) for 1 h.

Kindly advise the catalog no. of this isotype control.

Thanks in advance.

Abcam community

Verified customer

Asked on Jan 18 2013

Answer

Unfortunately, in few cases we cannot find the connection between the antibody used in a publication and the antibody used in our catalog, especially as we are trying to link this kind of information to the datasheets.
However, as we have only two matching isotypes in this case, and both differ only in the amount we sell:
https://www.abcam.com/Rabbit-IgG-monoclonal-SP137-isotype-control-ab125938.html https://www.abcam.com/Rabbit-IgG-monoclonal-SP137-isotype-control-ab125938.html.
https://www.abcam.com/Rabbit-IgG-monoclonal-SP137-isotype-control-pre-diluted-ab128142.html https://www.abcam.com/Rabbit-IgG-monoclonal-SP137-isotype-control-pre-diluted-ab128142.html.

Abcam Scientific Support

回复于 Jan 18 2013

Question

Phone call requesting the concentration of the Anti-MERTK antibody [Y323] (ab52968)

Abcam community

Verified customer

Asked on Oct 05 2012

Answer

Thank you for contacting us and your interest in our products.

I can now confirm that the concentration of the anti-MERTK antibody [Y323] (ab52968), of the lot number GR54920-5 is 0.1180 mg/ml.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Abcam Scientific Support

回复于 Oct 05 2012

Question

Hi, please it is very urgent,
can you reply?
Kind regards

Abcam community

Verified customer

Asked on Jun 05 2012

Answer

Thank you for contacting us and sorry for the delay of our reply.
Please find below the reply I just received from the source of the anti-MERTK antibody [Y323], ab52968:
> "Sorry for the delay in response. As stated previously, the anti-MERTK antibody ab52968 displayed clear membranous and some cytoplasmic IHC staining of human lymphoma tissue.
> Yes, it is true despite the region of the immunogen.
> The image is from formalin-fixed paraffin-embedded tissue, and we performed in-house testing.
> Although we do not have a paper for IHC, the antibody's specificity is demonstrated in WB and IF.
> Unfortunately, that is all the information we could find."
I appreciate the time you have spent on these experiments and would be pleased to arrange, if you agree, a replacement in compensation. The lot we currently have in stock is GR54920. Let me know if you received a different lot so I could send this one to you. Also, could you please inform me when the antibody was ordered and if it was ordered via a distributor?
Alternatively I can send you another anti-MERTK antibody that could maybe confirm the results you obtained with ab52968. The link to the list of the 9 anti-MERTK we have is: https://www.abcam.com/index.html?t=17227&pt=1
We would like to investigate this issue much further, so could you please provide some information about your protocol by filling in the attached questionnaire?
I look forward to your reply and I hope this case will be resolved as quickly as possible.
Thank you for your cooperation.

Abcam Scientific Support

回复于 Jun 05 2012

Question

Hi, please it is very urgent,
can you reply?

Abcam community

Verified customer

Asked on Jun 04 2012

Answer

I just wanted to let you know that we are still waiting for a reply from the originator of this product. I have sent a reminder and I hope we will receive the details about the IHC image you requested in the very near future.
I am very sorry for the delay. I will forward to you the information as soon as I receive it. Thank you for your patience.

Abcam Scientific Support

回复于 Jun 04 2012

Question

Esteemed Company,
I am working with abcam Antibody ab52968 (for Mertk).
Looking at image reported in your data sheet regarding IHC result, I can see a staining that not appear to be membranous as expected. Can you explain me the result?
Thank you very much.
Best regards.

Abcam community

Verified customer

Asked on May 09 2012

Answer

Thank you for contacting us. I apologise for the waiting.
Anti-MERTK antibody [Y323] (ab52968) displayed clear membranous and some cytoplasmic IHC staining of human lymphoma tissue (see larger image attached).
The Human Protein Atlas states “Most normal tissues showed moderate to strong cytoplasmic positivity with a granular pattern” (see image from the link below).
http://www.proteinatlas.org/ENSG00000153208
I also found another example of MER cytoplasmic staining in IHC (attached).
I hope this information was helpful. Please do not hesitate to contact us back for further assistance.

Abcam Scientific Support

回复于 May 09 2012

Question

The datasheet for this product states that it recognizes both human and mouse however, all of the experimental data provided are performed with human tissues and cells. We need an antibody against mouse MerTK that has been shown to be successful for flow cytometry. If we order this antibody (along with the recomended secondary needed for FC) and we are unsuccessful with mouse samples using flow cytometry, are there any quality assurances? Will you provide supplemental antibody or refund? We are a small laboratory, so we have to stretch our funds. I don't want an investment in this product to be a loss of both valuable time and research funds.

Abcam community

Verified customer

Asked on Oct 21 2011

Answer

Thank you for contacting Abcam. The antibody, ab52968, anti-MERTK antibody [Y323], is covered under our Abpromise, which means that if the antibody does not work as stated on the data sheet and if our support specialist cannot help resolve the issue, then we will provide you with a free of charge replacement or a refund. In this case it is guranteed to work in flow cytometry in mouse tissue. I hope this helps assure about our products and our guaranteed . If there is anything else I can do, please let me know.

Abcam Scientific Support

回复于 Oct 21 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (51)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MERTK antibody [Y323]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MERTK antibody [Y323]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MERTK antibody [Y323]

Western blot abreview for Anti-MERTK antibody [Y323]