Anti-Menin抗体(ab2605)
Key features and details
- Rabbit polyclonal to Menin
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Menin抗体
参阅全部 Menin 一抗 -
描述
兔多克隆抗体to Menin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Dog -
免疫原
Synthetic peptide (Human) - which represents a portion of the C-terminus of human MEN1.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯化说明
Affinity purified using the immunising peptideimmobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
WB | 1/10000 - 1/25000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).Can be blocked with Recombinant Menin protein (ab114387). | |
IP | Use at 2-10 µg/mg of lysate. |
靶标
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功能
Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair. -
组织特异性
Ubiquitous. -
疾病相关
Defects in MEN1 are the cause of familial multiple endocrine neoplasia type I (MEN1) [MIM:131100]. Autosomal dominant disorder characterized by tumors of the parathyroid glands, gastro-intestinal endocrine tissue, the anterior pituitary and other tissues. Cutaneous lesions and nervous-tissue tumors can exist. Prognosis in MEN1 patients is related to hormonal hypersecretion by tumors, such as hypergastrinemia causing severe peptic ulcer disease (Zollinger-Ellison syndrome, ZES), primary hyperparathyroidism, and acute forms of hyperinsulinemia.
Defects in MEN1 are the cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation. - Information by UniProt
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数据库链接
- Entrez Gene: 539431 Cow
- Entrez Gene: 483758 Dog
- Entrez Gene: 4221 Human
- Entrez Gene: 17283 Mouse
- Entrez Gene: 29417 Rat
- Omim: 613733 Human
- SwissProt: Q0P5I0 Cow
- SwissProt: A2SXS5 Dog
see all -
别名
- MEA 1 antibody
- MEA1 antibody
- MEN 1 antibody
see all
图片
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All lanes : Anti-Menin antibody (ab2605) at 0.04 µg/ml
Lane 1 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 68 kDaSamples in NETN lysis buffer.
Exposure time: 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)
Immunohistochemical analysis of human lung carcinoma tissue labeling Menin with ab2605 at 1 µg/ml. Detection: DAB.
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Menin was imunnoprecipitated from 1.0 mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab2605 at 6 µg/ml. Western blot was perfomed from the immunoprecipitate using ab2506 at 0.04 µg/ml.
Lane 1 and 2: 20% of ab2605 IP in HEK-293T whole cell lysate
Lane 3: Control IgG
Exposure time: 1 second
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)
Immunohistochemical analysis of human breast carcinoma tissue labelling Menin with ab2605 at 1µg/ml. Detection: DAB.
实验方案
文献 (19)
ab2605 被引用在 19 文献中.
- Yoshiji S et al. Alu-Mediated MEN1 Gene Deletion and Loss of Heterozygosity in a Patient with Multiple Endocrine Neoplasia Type 1. J Endocr Soc 4:bvaa051 (2020). PubMed: 32715270
- Lines KE et al. MiR-15a/miR-16-1 expression inversely correlates with cyclin D1 levels in Men1 pituitary NETs. J Endocrinol N/A:N/A (2018). PubMed: 30389902
- Uraki S et al. Atypical pituitary adenoma with MEN1 somatic mutation associated with abnormalities of DNA mismatch repair genes; MLH1 germline mutation and MSH6 somatic mutation. Endocr J 64:895-906 (2017). PubMed: 28701629
- Shell J et al. SomaticVHLMutation in a Patient With MEN1-Associated Metastatic Pancreatic Neuroendocrine Tumor Responding to Sunitinib Treatment: A Case Report. J Endocr Soc 1:1124-1134 (2017). PubMed: 29264567
- Lines KE et al. A MEN1 pancreatic neuroendocrine tumour mouse model under temporal control. Endocr Connect 6:232-242 (2017). IHC ; Mouse . PubMed: 28420716