Anti-MELK抗体[2G2] (ab129373)

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Key features and details

  • Mouse monoclonal [2G2] to MELK
  • Suitable for: ELISA, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-MELK抗体[2G2]
    参阅全部 MELK 一抗

  • 描述

    小鼠单克隆抗体[2G2] to MELK

  • 宿主

    Mouse

  • 经测试应用

    适用于: ELISA, IHC-P, Flow Cyt, ICC/IFmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis

  • 免疫原

    Synthetic peptide:

    C-VYKRLVEDILSSCKV

    (with a Cysteine residue linker), corresponding to C terminal amino acids 637-651 of Human MELK (Q14680).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • HepG2 cells and MCF-7 cells. Human liver cancer tissue.

  • 常规说明

    This product was changed from ascites to supernatant. Lot no’s high than GR248807-19 are from Tissue Culture Supernatant

性能

应用

Our Abpromise guarantee covers the use of ab129373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.

靶标

  • 功能

    Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.

  • 组织特异性

    Expressed in placenta, kidney, thymus, testis, ovary and intestine.

  • 序列相似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.

  • 翻译后修饰

    Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AI327312 antibody
      • hMELK antibody
      • HPK 38 antibody
      • hPK38 antibody
      • KIAA0175 antibody
      • Likely ortholog of maternal embryonic leucine zipper kinase antibody
      • Maternal embryonic leucine zipper kinase antibody
      • MELK antibody
      • MELK_HUMAN antibody
      • mKIAA0175 antibody
      • MPK38 antibody
      • OTTHUMP00000021377 antibody
      • OTTHUMP00000046113 antibody
      • pEg3 kinase antibody
      • Protein kinase Eg3 antibody
      • Protein kinase PK38 antibody
      • RP23 382O11.1 antibody
      • Tyrosine protein kinase MELK antibody
      see all

    图片

    • Flow Cytometry - Anti-MELK antibody [2G2] (ab129373)
      Flow Cytometry - Anti-MELK antibody [2G2] (ab129373)
      ab129373 at 1/200 dilution (green) or negative control (red) staining MELK in MCF-7 cells by flow cytometry.
    • Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373)
      Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373)
      ab129373 at 1/200 dilution staining MELK (green) in HepG2 cells by immunofluorescence. DNA is stained with DRAQ fluorescent dye (blue) and actin filaments with Alexa Fluor-555 phalloidin (red).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
      ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
      ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.

    文献

    发表研究结果有使用 ab129373？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab129373 被引用在 1 文献中.

    • Meel MH  et al. MEK/MELK inhibition and blood-brain barrier deficiencies in atypical teratoid/rhabdoid tumors. Neuro Oncol 22:58-69 (2020). PubMed: 31504799

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