Anti-MELK抗体[2G2] (ab129373)
![Flow Cytometry - Anti-MELK antibody [2G2] (ab129373)](https://www.abcam.cn/ps/products/129/ab129373/Images/ab129373-143568-anti-melk-antibody-2g2-flow-cytometry.jpg "Flow Cytometry - Anti-MELK antibody [2G2] (ab129373)")
Key features and details
- Mouse monoclonal [2G2] to MELK
- Suitable for: IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-MELK抗体[2G2]
参阅全部 MELK 一抗 -
描述
小鼠单克隆抗体[2G2] to MELK -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
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免疫原
Synthetic peptide:
C-VYKRLVEDILSSCKV
(with a Cysteine residue linker), corresponding to C terminal amino acids 637-651 of Human MELK (Q14680). -
阳性对照
- HepG2 cells and MCF-7 cells. Human liver cancer tissue.
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常规说明
This product was changed from ascites to supernatant. Lot no’s high than GR248807-19 are from Tissue Culture Supernatant
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
2G2 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab129373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/200 - 1/1000. | |
| Flow Cyt | 1/200 - 1/400. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | 1/200 - 1/1000. |
靶标
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功能
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis. -
组织特异性
Expressed in placenta, kidney, thymus, testis, ovary and intestine. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 9833 Human
- Entrez Gene: 17279 Mouse
- Omim: 607025 Human
- SwissProt: Q14680 Human
- SwissProt: Q61846 Mouse
- Unigene: 184339 Human
- Unigene: 268668 Mouse
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别名
- AI327312 antibody
- hMELK antibody
- HPK 38 antibody
see all
图片
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Flow Cytometry - Anti-MELK antibody [2G2] (ab129373)ab129373 at 1/200 dilution (green) or negative control (red) staining MELK in MCF-7 cells by flow cytometry.
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![Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373)](https://www.abcam.cn/ps/products/129/ab129373/Images/ab129373-143567-anti-melk-antibody-2g2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373)ab129373 at 1/200 dilution staining MELK (green) in HepG2 cells by immunofluorescence. DNA is stained with DRAQ fluorescent dye (blue) and actin filaments with Alexa Fluor-555 phalloidin (red). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)](https://www.abcam.cn/ps/products/129/ab129373/Images/ab129373-143566-anti-melk-antibody-2g2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)](https://www.abcam.cn/ps/products/129/ab129373/Images/ab129373-143565-anti-melk-antibody-2g2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.
实验方案
数据表及文件
文献 (1)
ab129373 被引用在 1 文献中.
- Meel MH et al. MEK/MELK inhibition and blood-brain barrier deficiencies in atypical teratoid/rhabdoid tumors. Neuro Oncol 22:58-69 (2020). PubMed: 31504799
