Anti-Melanophilin抗体(ab2716)
Key features and details
- Goat polyclonal to Melanophilin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Melanophilin抗体
参阅全部 Melanophilin 一抗 -
描述
山羊多克隆抗体to Melanophilin -
宿主
Goat -
特异性
ab2716 recognises Melanophilin. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Mouse Melanophilin aa 577-590 (C terminal).
Sequence:ARHIFAKPVMAQQP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab2716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 65 kDa. 1 hour primary incubation is recommended for this product. |
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| ICC/IF | Use a concentration of 0.5 µg/ml. |
靶标
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功能
Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A. -
疾病相关
Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3) [MIM:609227]. GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations. -
序列相似性
Contains 1 FYVE-type zinc finger.
Contains 1 RabBD (Rab-binding) domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 171531 Mouse
- Entrez Gene: 316620 Rat
- SwissProt: Q91V27 Mouse
- Unigene: 105208 Mouse
- Unigene: 124575 Rat
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别名
- 2210418F23Rik antibody
- 5031433I09Rik antibody
- AW228792 antibody
see all
图片
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Western blot - Anti-Melanophilin antibody (ab2716)All lanes : Anti-Melanophilin antibody (ab2716) at 0.5 µg/ml
Lane 1 : total melanocyte PNS (200ug per lane) of wild-type
Lane 2 : total melanocyte PNS (200ug per lane) of leaden (Mlph null) mice
Predicted band size: 65 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
ab2716 staining (3µ g/ml) of Jurkat lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence. -
Immunocytochemistry/ Immunofluorescence - Anti-Melanophilin antibody (ab2716)Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.
实验方案
数据表及文件
文献 (1)
ab2716 被引用在 1 文献中.
- Kudo M et al. Bifunctional effects of O-methylated flavones from Scutellaria baicalensis Georgi on melanocytes: Inhibition of melanin production and intracellular melanosome transport. PLoS One 12:e0171513 (2017). PubMed: 28182699
