重组Anti-Melanoma gp100抗体[EP4863(2)] (ab137078)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4863(2)] to Melanoma gp100
- Suitable for: WB, IHC-P, IP, Flow Cyt (Intra), IA, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Melanoma gp100抗体[EP4863(2)]
参阅全部 Melanoma gp100 一抗 -
描述
兔单克隆抗体[EP4863(2)] to Melanoma gp100 -
宿主
Rabbit -
特异性
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
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经测试应用
适用于: WB, IHC-P, IP, Flow Cyt (Intra), IA, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human Melanoma gp100 aa 1-100. The exact sequence is proprietary.
Database link: P40967 -
阳性对照
- WB: B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysate and Human melanoma lysate. IHC: Human melanoma tissue. ICC/IF: B16-F0 (Mouse melanoma epithelial cell-like) cell line. Flow Cyt (intra): B16-F0 (Mouse melanoma epithelial cell-like) cell line. IP: B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysate.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP4863(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)
- Biotin Anti-Melanoma gp100 antibody [EP4863(2)] (ab204674)
- HRP Anti-Melanoma gp100 antibody [EP4863(2)] (ab205469)
- Alexa Fluor® 647 Anti-Melanoma gp100 antibody [EP4863(2)] (ab246730)
- PE Anti-Melanoma gp100 antibody [EP4863(2)] (ab246731)
- Alexa Fluor® 568 Anti-Melanoma gp100 antibody [EP4863(2)] (ab312941)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137078于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
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IHC-P | (5) |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. |
IP |
1/30.
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Flow Cyt (Intra) |
1/40.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IA |
1/50.
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ICC/IF | (2) |
1/50.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 70 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. |
IP
1/30. |
Flow Cyt (Intra)
1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IA
1/50. |
ICC/IF
1/50. |
靶标
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功能
Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. -
组织特异性
Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. -
序列相似性
Belongs to the PMEL/NMB family.
Contains 1 PKD domain. -
结构域
The RPT domain is essential for the generation of the fibrillar matrix of melanosomes.
The lumenal domain is necessary for correct processing and trafficking to melanosomes. -
翻译后修饰
A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides.
N-glycosylated. O-glycosylated; contains sialic acid. -
细胞定位
Secreted and Endoplasmic reticulum membrane. Golgi apparatus. Melanosome. Endosome > multivesicular body. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes. - Information by UniProt
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数据库链接
- Entrez Gene: 6490 Human
- Entrez Gene: 20431 Mouse
- Omim: 155550 Human
- SwissProt: P40967 Human
- SwissProt: Q60696 Mouse
- Unigene: 95972 Human
- Unigene: 16756 Mouse
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别名
- 95 kDa melanocyte specific secreted glycoprotein antibody
- 95 kDa melanocyte-specific secreted glycoprotein antibody
- D12S53E antibody
see all
图片
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ab137078 immunoprecipitating Melanoma gp100. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.
Lane 1: B16-F0 (mouse melanoma epithelial cell-like) whole cell lysate 10ug
Lane 2: B16-F0 (mouse melanoma epithelial cell-like) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab137078 in B16-F0 whole cell lysateExposure time: 1 second
Observed MW: 85-100 KDa
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Overlay histogram showing B16-F0 (Mouse skin melanoma cell) cells stained with ab137078 (red line). The cells were fixed with 4% paraformaldehydeand then permeabilized with 90% methanol. The cells were then incubated with ab137078 at 1/40 dilution. The secondary antibody used wasGoat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution. Isotype control antibody (black line) was Rabbit monoclonal IgG (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody.
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ab137078 staining Melanoma gp100 in B16-F0 (mouse skin) cells by ICC (Immunocytochemistry). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) ab195889 was used as a counterstain at a dilution of 1/200. DAPI was used as a nuclear counterstain.
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ab137078 staining Melanoma gp100 in human melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250 for 30 minutes at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
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Anti-Melanoma gp100 antibody [EP4863(2)] (ab137078) at 1/1000 dilution + Human melanoma lysate 15µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 70 kDaExposure time: 18-0 seconds
Observed MW: 85-100 KDa
The molecular weights observed are consistent with what have been described in PMID: 16492709 and PMID: 7961886
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Anti-Melanoma gp100 antibody [EP4863(2)] (ab137078) at 1/10000 dilution + B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 70 kDaExposure time: 50 seconds
Observed MW: 85-100 KDa
The molecular weights observed are consistent with what have been described in PMID: 16492709 and PMID: 7961886
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (44)
ab137078 被引用在 44 文献中.
- Clements D et al. Cross talk between LAM cells and fibroblasts may influence alveolar epithelial cell behavior in lymphangioleiomyomatosis. Am J Physiol Lung Cell Mol Physiol 322:L283-L293 (2022). PubMed: 34936509
- Yang H et al. Non-invasive detection and complementary diagnosis of liver metastases via chemokine receptor 4 imaging. Cancer Gene Ther 29:1827-1839 (2022). PubMed: 35145271
- Tanwar J et al. Mitofusin-2 Negatively Regulates Melanogenesis by Modulating Mitochondrial ROS Generation. Cells 11:N/A (2022). PubMed: 35203350
- Sultan F et al. Temporal analysis of melanogenesis identifies fatty acid metabolism as key skin pigment regulator. PLoS Biol 20:e3001634 (2022). PubMed: 35584084
- Vittoria MA et al. Inactivation of the Hippo tumor suppressor pathway promotes melanoma. Nat Commun 13:3732 (2022). PubMed: 35768444