Anti-Melanoma抗体[HMB45 + M2-7C10 + M2-9E3] (ab732)
Key features and details
- Mouse monoclonal [HMB45 + M2-7C10 + M2-9E3] to Melanoma
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2b
概述
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产品名称
Anti-Melanoma抗体[HMB45 + M2-7C10 + M2-9E3]
参阅全部 Melanoma 一抗 -
描述
小鼠单克隆抗体[HMB45 + M2-7C10 + M2-9E3] to Melanoma -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
HMB45 - Pigmented melanoma metastases from LN MART-1 - Recombinant human MART-1 protein
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表位
HMB45 and MART-1 -
阳性对照
- IHC-P: Metastatic melanoma in lymph node.
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常规说明
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail is HMB45 + two different clones of MART-1[M2-7C10] + [M2-9E3]. HMB34 isotype: IgG1/kappa and both clones of MART-1: IgG2b + IgG2b
HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones M2-7C10 and M2-9E3. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.
This product was changed from ascites to tissue culture supernatant on [14/07/17]. The following lots are from ascites and are still in stock as of [14/07/17] – [GR313023, GR3175007]. Lot numbers higher than [GR313023] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.099% Sodium azide
Constituents: 0.9% Proprietary component, 99% Water -
Concentration information loading...
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纯度
Tissue culture supernatant -
Primary antibody说明
HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones DT101 and BC199. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener. -
克隆
单克隆 -
克隆编号
HMB45 + M2-7C10 + M2-9E3 -
骨髓瘤
unknown -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
1/100 - 1/200.
The staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. |
说明 |
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IHC-P
1/100 - 1/200. The staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. |
靶标
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相关性
Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites. -
细胞定位
Membrane; Single-pass membrane protein -
数据库链接
- Entrez Gene: 77836 Mouse
- Entrez Gene: 293890 Rat
- Omim: 605513 Human
- SwissProt: Q16655 Human
- SwissProt: Q2TA50 Mouse
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别名
- Antigen LB39 AA antibody
- Antigen SK29 AA antibody
- MART1 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab732 被引用在 21 文献中.
- Golan T et al. Adipocytes sensitize melanoma cells to environmental TGF-ß cues by repressing the expression of miR-211. Sci Signal 12:N/A (2019). PubMed: 31337739
- Li H et al. YAP/TAZ Activation Drives Uveal Melanoma Initiation and Progression. Cell Rep 29:3200-3211.e4 (2019). PubMed: 31801083
- Han N et al. Enhanced IL-34 expression in Nivolumab-resistant metastatic melanoma. Inflamm Regen 38:3 (2018). PubMed: 29515691
- Malcov-Brog H et al. UV-Protection Timer Controls Linkage between Stress and Pigmentation Skin Protection Systems. Mol Cell 72:444-456.e7 (2018). PubMed: 30401431
- Yanaki M et al. In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation. Integr Cancer Ther 17:646-653 (2018). PubMed: 29665734