Key features and details
- Mouse monoclonal [HMB45 + M2-7C10 + M2-9E3] to Melanoma
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2b
产品名称Anti-Melanoma抗体[HMB45 + M2-7C10 + M2-9E3]
参阅全部 Melanoma 一抗
描述小鼠单克隆抗体[HMB45 + M2-7C10 + M2-9E3] to Melanoma
经测试应用适用于: IHC-Pmore details
种属反应性与反应: Mouse, Human
HMB45 - Pigmented melanoma metastases from LN MART-1 - Recombinant human MART-1 protein
表位HMB45 and MART-1
- IHC-P: Metastatic melanoma in lymph node.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail is HMB45 + two different clones of MART-1[M2-7C10] + [M2-9E3]. HMB34 isotype: IgG1/kappa and both clones of MART-1: IgG2b + IgG2b
HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones M2-7C10 and M2-9E3. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.
This product was changed from ascites to tissue culture supernatant on [14/07/17]. The following lots are from ascites and are still in stock as of [14/07/17] – [GR313023, GR3175007]. Lot numbers higher than [GR313023] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.099% Sodium azide
Constituents: 0.9% Proprietary component, 99% Water
Concentration information loading...
纯度Tissue culture supernatant
Primary antibody说明HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones DT101 and BC199. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.
克隆编号HMB45 + M2-7C10 + M2-9E3
Our Abpromise guarantee covers the use of ab732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/200.
The staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail.
相关性Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.
细胞定位Membrane; Single-pass membrane protein
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ab732 被引用在 21 文献中.
- Golan T et al. Adipocytes sensitize melanoma cells to environmental TGF-ß cues by repressing the expression of miR-211. Sci Signal 12:N/A (2019). PubMed: 31337739
- Li H et al. YAP/TAZ Activation Drives Uveal Melanoma Initiation and Progression. Cell Rep 29:3200-3211.e4 (2019). PubMed: 31801083
- Han N et al. Enhanced IL-34 expression in Nivolumab-resistant metastatic melanoma. Inflamm Regen 38:3 (2018). PubMed: 29515691
- Malcov-Brog H et al. UV-Protection Timer Controls Linkage between Stress and Pigmentation Skin Protection Systems. Mol Cell 72:444-456.e7 (2018). PubMed: 30401431
- Yanaki M et al. In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation. Integr Cancer Ther 17:646-653 (2018). PubMed: 29665734