Anti-Melanoma抗体[HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)

Reviews (1)Q&A (1)References (6)

Key features and details

  • Mouse monoclonal [HMB45 + M2-7C10 + M2-9E3 + T311] to Melanoma
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Melanoma抗体[HMB45 + M2-7C10 + M2-9E3 + T311]
    参阅全部 Melanoma 一抗

  • 描述

    小鼠单克隆抗体[HMB45 + M2-7C10 + M2-9E3 + T311] to Melanoma

  • 宿主

    Mouse

  • 特异性

    The HMB45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas. MART-1 recognizes a protein of 18kDa, identified at MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown Tyrosinase to be a more sensitive marker when compared to HMB45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB45.

  • 经测试应用

    适用于: Flow Cyt, ICC/IF, IHC-P, IHC-Frmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    HMB45 - Pigmented melanoma metastases from LN MART-1 - Recombinant human MART-1 protein Tyrosinase - Recombinant tyrosinase protein

  • 阳性对照

    • Metastatic melanoma in lymph node.

  • 常规说明

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

    The combination of HMB45, MART-1 (M2-7C10 + M2-9E3) and Tyrosinase (T311) (SwissProt: P14679 Human, Omim: 606933 Human, Entrez Gene: 22173 Mouse, Entrez Gene: 308800 Rat) make this quadruple antibody cocktail a first-order pan melanoma screener, and may prove to be a valuable marker for melanoma metastasis in sentinel lymph nodes (see reference 3.).

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.30
    Constituents: PBS, Tissue culture supernatant

    Proprietary preservative that is not sodium azide or thimerosal, protein carrier.
  • Concentration information loading...

  • Primary antibody说明

    The combination of HMB45, MART-1 (DT101 + BC199) and Tyrosinase (T311) make this quadruple antibody cocktail a first-order pan melanoma screener, and may prove to be a valuable marker for melanoma metastasis in sentinel lymph nodes (see reference 3.).

  • 克隆

    单克隆

  • 克隆编号

    HMB45 + M2-7C10 + M2-9E3 + T311

  • 骨髓瘤

    unknown

  • 同种型

    IgG

  • 轻链类型

    kappa

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment, therefore we recommend you substitute an AEC or a Fast Red substrate protocol.
IHC-Fr 1/25 - 1/50. ABC method.

靶标

  • 相关性

    Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.

  • 细胞定位

    Membrane; Single-pass membrane protein

  • 数据库链接

    • 别名

      • Antigen LB39 AA antibody
      • Antigen SK29 AA antibody
      • MART1 antibody
      • melan A antibody
      • Melan A protein antibody
      • Melanoma antigen recognized by T-cells 1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)
      IHC image of ab733 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab733, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)

      ab733 staining Melanoma by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    • Flow Cytometry - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)
      Flow Cytometry - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)

      Overlay histogram showing MALME 3M cells stained with ab733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab733, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)
      Immunocytochemistry/ Immunofluorescence - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)This image is courtesy of an Abreview submitted by Hongwei Shao
      Immunofluorescence analysis of cultured Human melanoma cells, staining Melanoma with ab733.

      Cells were fixed with formaldehyde and blocked with blocking reagent for 30 minutes at room temperature. Samples were incubated with primary antibody (1/25 in blocking solution) for 1 hour at 37°C. An AlexaFluor®594-conjugated donkey anti-mouse polyclonal IgG (1/200) was used as the secondary antibody.

      See Abreview

    文献 (6)

    发表研究结果有使用 ab733？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab733 被引用在 6 文献中.

    • Li Z  et al. Robust Photodynamic Therapy Using 5-ALA-Incorporated Nanocomplexes Cures Metastatic Melanoma through Priming of CD4+CD8+ Double Positive T Cells. Adv Sci (Weinh) 6:1802057 (2019). PubMed: 30886812
    • Lin H  et al. Host expression of PD-L1 determines efficacy of PD-L1 pathway blockade-mediated tumor regression. J Clin Invest 128:805-815 (2018). PubMed: 29337305
    • Ness C  et al. Multicellular tumor spheroids of human uveal melanoma induce genes associated with anoikis resistance, lipogenesis, and SSXs. Mol Vis 23:680-694 (2017). PubMed: 29033534
    • Kundu S  et al. Heparanase Promotes Glioma Progression and Is Inversely Correlated with Patient Survival. Mol Cancer Res 14:1243-1253 (2016). PubMed: 27565180
    • Savla R  et al. Tumor-targeted responsive nanoparticle-based systems for magnetic resonance imaging and therapy. Pharm Res 31:3487-502 (2014). PubMed: 24919932
    • Giordano G  et al. The role of cervical smear in the diagnosis and management of extrauterine malignancies metastatic to the cervix: three case reports. Diagn Cytopathol 38:41-6 (2010). IHC-P ; Human . PubMed: 19626626

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    Phone call requesting concentration of antibody.

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    Answer

    Thank you for contacting us and your interest in our products.

    I am sorry for the delay in getting back to you. I can now confirm that the anti-Melanoma antibody [HMB45 + DT101 + BC199 + T311] (ab733) is composed of a combination of HMB45, MART-1 (DT101 + BC199) and Tyrosinase (T311) make this a quadruple antibody cocktail, a first-order pan melanoma screener. The concentration of each of the antibodies in the lot which you have received is as follows:

    HMB45 is 8.9µg/mL;
    MART-1 [m2-E93] is 22.2µg/mL;
    Melan A [M2-7C10] is 20µg/mL;
    Tyrosinase is 27µg/mL;

    I hope this has been helpful. If I can be of any further assistance, please do not hesitate to let me know.

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Melanoma antibody [HMB45 + DT101 + BC199 + T311]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (melanoma cell line)
    Specification
    melanoma cell line
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    Dakocytomation # X0909 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
    Read More

    Hongwei Shao

    Verified customer

    提交于 Jan 18 2013

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