Anti-MelanA抗体[DT101 + BC199] (ab731)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)](https://www.abcam.cn/ps/products/0/ab731/Images/ab731-205305-anti-melana-antibody-dt101-bc199-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)")
Key features and details
- Mouse monoclonal [DT101 + BC199] to MelanA
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-MelanA抗体[DT101 + BC199]
参阅全部 MelanA 一抗 -
描述
小鼠单克隆抗体[DT101 + BC199] to MelanA -
宿主
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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免疫原
Recombinant full length protein (Human).
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阳性对照
- Melanoma
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常规说明
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: PBS, Tissue culture supernatant
Proprietary preservative that is not sodium azide or thimerosal, protein carrier. -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
DT101 + BC199 -
骨髓瘤
unknown -
同种型
IgG2b -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| IHC-P |
Human
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| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
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| 说明 |
|---|
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method. |
靶标
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功能
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. -
组织特异性
Expression is restricted to melanoma and melanocyte cell lines and retina. -
翻译后修饰
Acylated. -
细胞定位
Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation. - Information by UniProt
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数据库链接
- Entrez Gene: 2315 Human
- Omim: 605513 Human
- SwissProt: Q16655 Human
- Unigene: 154069 Human
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别名
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)
Immunohistochemistry analysis of Melanoma tissue labeling MelanA with ab731.
数据表及文件
文献 (13)
ab731 被引用在 13 文献中.
- Semba T et al. Periostin antisense oligonucleotide suppresses bleomycin-induced formation of a lung premetastatic niche for melanoma. Cancer Sci 109:1447-1454 (2018). PubMed: 29498146
- Zeng H et al. Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation. Cancer Cell 34:56-68.e9 (2018). PubMed: 29990501
- Schwochow Thalmann D et al. The evolution of Sex-linked barring alleles in chickens involves both regulatory and coding changes in CDKN2A. PLoS Genet 13:e1006665 (2017). IHC-Fr ; Chicken . PubMed: 28388616
- Cao J et al. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest 127:349-364 (2017). PubMed: 27918305
- Mi W et al. YEATS2 links histone acetylation to tumorigenesis of non-small cell lung cancer. Nat Commun 8:1088 (2017). PubMed: 29057918
