重组Anti-MEK4/MKK4抗体[EP615Y] (ab33912)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP615Y] to MEK4/MKK4
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MEK4/MKK4抗体[EP615Y]
参阅全部 MEK4/MKK4 一抗 -
描述
兔单克隆抗体[EP615Y] to MEK4/MKK4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human MEK4/MKK4 (C terminal). The exact sequence is proprietary.
Database link: P45985 -
阳性对照
- WB: NIH/3T3 and HeLa whole cell lysates. Rat brain tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab33912 is a purified IgG. -
克隆
单克隆 -
克隆编号
EP615Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33912于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000. Predicted molecular weight: 44 kDa.
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说明 |
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WB
1/1000. Predicted molecular weight: 44 kDa. |
靶标
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功能
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). -
组织特异性
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. - Information by UniProt
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数据库链接
- Entrez Gene: 6416 Human
- Entrez Gene: 26398 Mouse
- Entrez Gene: 287398 Rat
- Omim: 601335 Human
- SwissProt: P45985 Human
- SwissProt: P47809 Mouse
- Unigene: 514681 Human
- Unigene: 412922 Mouse
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别名
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
see all
图片
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Anti-MEK4/MKK4 antibody [EP615Y] (ab33912) at 1/1000 dilution (Purified) + Rat brain lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDaBlocking buffer: 5% NFDM/TBST
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All lanes : Anti-MEK4/MKK4 antibody [EP615Y] (ab33912) at 1/4000 dilution (Purified)
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDaBlocking buffer: 5% NFDM/TBST
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Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: MEK4 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: A431 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target - ab33912 observed at 44 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalab33912 was shown to specifically react with MEK4 when MEK4 knockout samples were used. Wild-type and MEK4 knockout samples were subjected to SDS-PAGE. ab33912 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab33912 被引用在 8 文献中.
- Wu X et al. Amyloid precursor protein promotes the migration and invasion of breast cancer cells by regulating the MAPK signaling pathway. Int J Mol Med 45:162-174 (2020). PubMed: 31746365
- Shang GK et al. Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles. J Cachexia Sarcopenia Muscle 11:1104-1120 (2020). PubMed: 32096609
- Li Y et al. MiR-183-5p Promotes Proliferation, Metastasis and Angiogenesis in Breast Cancer Cells through Negatively Regulating Four and a Half LIM Protein 1. J Breast Cancer 23:355-372 (2020). PubMed: 32908787
- Niu J et al. Overexpressed microRNA-136 works as a cancer suppressor in gallbladder cancer through suppression of JNK signaling pathway via inhibition of MAP2K4. Am J Physiol Gastrointest Liver Physiol 317:G670-G681 (2019). PubMed: 31369289
- Pinto SK et al. Expression of microRNAs and target proteins in skeletal muscle of rats selectively bred for high and low running capacity. Am J Physiol Endocrinol Metab 313:E335-E343 (2017). PubMed: 28465283
- Lukey MJ et al. The oncogenic transcription factor c-Jun regulates glutaminase expression and sensitizes cells to glutaminase-targeted therapy. Nat Commun 7:11321 (2016). PubMed: 27089238
- Fey D et al. Signaling pathway models as biomarkers: Patient-specific simulations of JNK activity predict the survival of neuroblastoma patients. Sci Signal 8:ra130 (2015). PubMed: 26696630
- Wuestefeld T et al. A Direct in vivo RNAi screen identifies MKK4 as a key regulator of liver regeneration. Cell 153:389-401 (2013). IF, IHC ; Mouse . PubMed: 23582328