Key features and details
- Rabbit polyclonal to MEK1 (phospho T286)
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
产品名称Anti-MEK1 (phospho T286)抗体
参阅全部 MEK1 一抗
描述兔多克隆抗体to MEK1 (phospho T286)
经测试应用适用于: IP, IHC-Pmore details
种属反应性与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Elephant
Synthetic peptide corresponding to amino acids surrounding Threonine 286 of Human MEK1 when the Threonine residue is phosphorylated (NP_002746.1).
- HeLa whole cell lysate, treated with nocodazole.
存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab129431 was affinity purified using an epitope specific to Phospho MEK1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab129431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 2 - 10 µg/ml.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
功能Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
组织特异性Widely expressed, with extremely low levels in brain.
疾病相关Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
翻译后修饰Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Information by UniProt
- Dual specificity mitogen activated protein kinase kinase 1 antibody
- Dual specificity mitogen-activated protein kinase kinase 1 antibody
- ERK activator kinase 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling MEK1 (phospho T286) with ab129431 at 1/1000 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MEK1 (phospho T286) with ab129431 at 1/5000 (0.2µg/ml). Detection: DAB.
Detection of MEK1 by Western Blot of Immunprecipitate.
ab129431 at 1µg/ml (lanes 1-4) or Control IgG (lanes 5-6)staining MEK1 in HeLa whole cell lysate immunoprecipitated using ab129431 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 1: HeLa whole cell lysate, mock treated.
Lane 2: HeLa whole cell lysate, nocodazole treated.
Lane 3: Immunoprecipitation of MEK1 (phospho T286) from mock treated HeLa whole cell lysate.
Lane 4: Immunoprecipitation of MEK1 (phospho T286) from nocodazole treated HeLa whole cell lysate.
Lane 5: HeLa whole cell lysate, mock treated.
Lane 6: HeLa whole cell lysate, nocodazole treated.
Predicted band size : 43 kDa.
Detection: Chemiluminescence with exposure time 10 seconds.