Anti-MED13/GCT抗体(ab229717)
Key features and details
- Rabbit polyclonal to MED13/GCT
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MED13/GCT抗体
参阅全部 MED13/GCT 一抗 -
描述
兔多克隆抗体to MED13/GCT -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment within Human MED13/GCT (internal sequence). The exact sequence is proprietary.
Database link: Q9UHV7 -
阳性对照
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab229717于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 239 kDa.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 239 kDa. |
靶标
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相关性
MED13 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 9969 Human
- Entrez Gene: 327987 Mouse
- Entrez Gene: 303403 Rat
- Omim: 603808 Human
- SwissProt: Q9UHV7 Human
- SwissProt: Q5SWW4 Mouse
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别名
- 1110067M05Rik antibody
- Activator-recruited cofactor 250 kDa component antibody
- ARC250 antibody
see all
图片
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All lanes : Anti-MED13/GCT antibody (ab229717) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 239 kDa5% SDS-PAGE gel.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab229717 尚未被引用在任何文献中。