Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary cortical neurons)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Treatment
50mM KCl or NaCl for 30min
Specification
Primary cortical neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS with 5% BSA and 0.1% Tween 20
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 55 kDa Non-specific: NA kDa
Positive control
Primary cortical neurons from cdk5 WT and knockout mice treated with 50mM NaCl or KCl for 30min.
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提交于 Dec 12 2019