MDR Assay试剂盒(Fluorometric) (ab112142)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells
概述
-
产品名称
MDR Assay试剂盒(Fluorometric)
参阅全部 MDR 试剂盒 -
检测方法
Fluorescent -
样品类型
Adherent cells, Suspension cells -
检测类型
Quantitative -
检测时间
1h 0m -
种属反应性
与反应: Mammals, Other species -
产品概述
Multi drug resistance (MDR) is a major factor in the failure of many forms of chemotherapy. In the past few years it has become widely accepted that the resistance to chemotherapy correlates with the overexpression of at least two ATP dependent drug efflux pumps. These cell membrane proteins, called P-glycoprotein (Pgp, MDR1), and multidrug resistance associated protein (MRP1) are members of the ABC transporter family. Abcam's Fluorimetric MDR Assay Kits use a fluorescent MDR indicator for assaying these two MDR pump activities. This hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped in cells. Following a short incubation, the intracellular free dye concentration can increase significantly. In the MDR1 and/or MRP1-expressing cells this dye is extruded by the MDR transporters, thus decreasing the cellular fluorescence intensity. However, when its extrusion is blocked by an agent that interferes with the MDR1 and/or MRP1 pump-activity, its cellular fluorescence intensity increases significantly. Abcam's Fluorimetric MDR Assay Kits are ideal for high throughput screening of MDR pump inhibitors or identifying the cells that have high level of MDR pump activities.
Visit our FAQs page for tips and troubleshooting. -
说明
Store ab112142 dessicated.
-
平台
Microplate reader
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 10 x 96 tests Assay Buffer 1 x 10ml 1 x 100ml DMSO 1 x 100µl 1 x 300µl MDR Sensor 1 vial 1 vial -
研究领域
图片
-
Effect of Cyclosporin A on the inhibition of P-gp pump in MCF7/ADR cells. The increased concentration of Cyclosporin A resulted in an increase in fluorescence signal caused by the inhibition of P-gp pump which enhanced the intracellular accumulation of MDR indicator dye. The EC50 = 2.4 µM (measured with the kit) is similar to the value reported in the literature.
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab112142 被引用在 6 文献中.
- Ahn JH et al. Effect of Rumex Acetosa Extract, a Herbal Drug, on the Absorption of Fexofenadine. Pharmaceutics 12:N/A (2020). PubMed: 32545588
- He H et al. Perimitochondrial Enzymatic Self-Assembly for Selective Targeting the Mitochondria of Cancer Cells. ACS Nano 14:6947-6955 (2020). PubMed: 32383849
- Vascellari S et al. Cisplatin, glutathione and the third wheel: a copper-(1,10-phenanthroline) complex modulates cisplatin-GSH interactions from antagonism to synergism in cancer cells resistant to cisplatin. RSC Adv 9:5362-5376 (2019). PubMed: 35515894
- Wang X et al. Extracellular ATP, as an energy and phosphorylating molecule, induces different types of drug resistances in cancer cells through ATP internalization and intracellular ATP level increase. Oncotarget 8:87860-87877 (2017). PubMed: 29152126
- Wang S et al. Schisandrin B reverses doxorubicin resistance through inhibiting P-glycoprotein and promoting proteasome-mediated degradation of survivin. Sci Rep 7:8419 (2017). PubMed: 28827665
- Wang S et al. Evodiamine synergizes with doxorubicin in the treatment of chemoresistant human breast cancer without inhibiting P-glycoprotein. PLoS One 9:e97512 (2014). Functional Studies . PubMed: 24830744