重组Anti-MDMX/MDM4抗体[BL-3-2F2] (ab243859)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-3-2F2] to MDMX/MDM4
- Suitable for: WB, IP
- Reacts with: Human
概述
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产品名称
Anti-MDMX/MDM4抗体[BL-3-2F2]
参阅全部 MDMX/MDM4 一抗 -
描述
兔单克隆抗体[BL-3-2F2] to MDMX/MDM4 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human MDMX/MDM4 aa 125-175. The exact sequence is proprietary. NP_002384.2 and Gene ID 4194.
Database link: O15151 -
阳性对照
- WB: Jurkat, 293T, HeLa and A549 cell lysates. IP: Jurkat cell lysate.
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常规说明
This product is sold under License from Bethyl Laboratories, Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
Cell culture supernatant was harvested from mammalian cells expressing recombinant rabbit monoclonal antibody. -
克隆
单克隆 -
克隆编号
BL-3-2F2 -
同种型
IgG -
研究领域
相关产品
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab243859于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000.
|
|
IP |
Use at an assay dependent concentration.
Use 20µg/mg lysate. |
说明 |
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WB
1/1000. |
IP
Use at an assay dependent concentration. Use 20µg/mg lysate. |
靶标
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功能
Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. -
组织特异性
Expressed in all tissues tested with high levels in thymus. -
序列相似性
Belongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain. -
结构域
Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2. -
翻译后修饰
Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4194 Human
- Omim: 602704 Human
- SwissProt: O15151 Human
- Unigene: 497492 Human
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别名
- DKFZp781B1423 antibody
- Double minute 4 antibody
- Double minute 4 human homolog of p53 binding protein antibody
see all
图片
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MDMX/MDM4 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) using ab243859 at 20µg/mL. Western blot was performed from the immunoprecipitate using ab243859 at 1/100 dilution.
Lane 1: ab243859 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP.Chemiluminescence with an exposure time of 75 seconds.
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All lanes : Anti-MDMX/MDM4 antibody [BL-3-2F2] (ab243859)
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab243859 被引用在 1 文献中.
- Ananthapadmanabhan V et al. Milademetan is a highly potent MDM2 inhibitor in Merkel cell carcinoma. JCI Insight 7:N/A (2022). PubMed: 35801592