Anti-MDM2抗体[SMP 14] (ab3110)

Reviews (7)Q&A (9)References (17)
Western blot - Anti-MDM2 antibody [SMP 14] (ab3110)

    Key features and details

    • Mouse monoclonal [SMP 14] to MDM2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-MDM2抗体[SMP 14]
      参阅全部 MDM2 一抗

    • 描述

      小鼠单克隆抗体[SMP 14] to MDM2

    • 宿主

      Mouse

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • 免疫原

      Synthetic peptide corresponding to Human MDM2 aa 150-250. Cross-linked to KLH using glutaldehyde.

    • 表位

      Amino acids 154 - 167.

    • 阳性对照

      • HepG2 cell lysate (treated with 10uM Nutlin-3a for 24hrs)

    • 常规说明

      This antibody clone is manufactured by Abcam.

      If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    性能

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3110 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    WB
    Human
    应用 Ab评论 说明
    WB (3)
    Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
    说明
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.

    靶标

    • 功能

      E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways.

    • 组织特异性

      Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.

    • 疾病相关

      Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.

    • 序列相似性

      Belongs to the MDM2/MDM4 family.
      Contains 1 RanBP2-type zinc finger.
      Contains 1 RING-type zinc finger.
      Contains 1 SWIB domain.

    • 结构域

      Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.

    • 翻译后修饰

      Phosphorylated in response to ionizing radiation in an ATM-dependent manner.
      Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiquitinated by USP7; leading to stabilize it.

    • 细胞定位

      Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • ACTFS antibody
        • Double minute 2 protein antibody
        • E3 ubiquitin-protein ligase Mdm2 antibody
        • Hdm 2 antibody
        • Hdm2 antibody
        • HDMX antibody
        • MDM 2 antibody
        • MDM2 antibody
        • MDM2 oncogene E3 ubiquitin protein ligase antibody
        • Mdm2 p53 E3 ubiquitin protein ligase homolog antibody
        • Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody
        • MDM2_HUMAN antibody
        • MDM2BP antibody
        • Mouse Double Minute 2 antibody
        • MTBP antibody
        • Murine Double Minute Chromosome 2 antibody
        • Oncoprotein Mdm2 antibody
        • p53 Binding Protein Mdm2 antibody
        • p53-binding protein Mdm2 antibody
        • Ubiquitin protein ligase E3 Mdm2 antibody
        • Ubiquitin protein ligase E3 Mdm2 antibody
        see all

      图片

      • Western blot - Anti-MDM2 antibody [SMP 14] (ab3110)
        Western blot - Anti-MDM2 antibody [SMP 14] (ab3110)
        All lanes : Anti-MDM2 antibody [SMP 14] (ab3110) at 2 µg/ml

        Lane 1 : HepG2 cell lysate
        Lane 2 : HepG2 treated with 10uM Nutlin-3a for 24hrs cell lysate

        Lysates/proteins at 20 µg per lane.

        Performed under reducing conditions.

        Predicted band size: 55 kDa
        Observed band size: 75 kDa
        why is the actual band size different from the predicted?



        Lanes 1 - 2: Merged signal (red and green). Green - ab3110 observed at 75 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

        ab3110 was shown to react with MDM2 in cell lysates subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab3110 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at 2 µg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      文献 (17)

      发表研究结果有使用 ab3110？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab3110 被引用在 17 文献中.

      • Inoue C  et al. Renal Epithelioid Angiomyolipoma Undergoing Aggressive Clinical Outcome: The MDM2 Expression in Tumor Cells of Two Cases. Tohoku J Exp Med 247:119-127 (2019). PubMed: 30799331
      • Patel N  et al. HDAC2 Regulates Site-Specific Acetylation of MDM2 and Its Ubiquitination Signaling in Tumor Suppression. iScience 13:43-54 (2019). PubMed: 30818224
      • Wang B  et al. The expression of mouse double minute 2 homolog and P73 had no correlation with growth arrest DNA damage-inducible gene 45a in patients with non-small-cell lung carcinoma: A STROBE-compliant study. Medicine (Baltimore) 98:e17944 (2019). PubMed: 31860949
      • Moghadam FH  et al. Ferulic Acid exerts concentration-dependent anti-apoptotic and neuronal differentiation-inducing effects in PC12 and mouse neural stem cells. Eur J Pharmacol 841:104-112 (2018). PubMed: 30332611
      • Chen W  et al. Overexpression of GRIM-19 accelerates radiation-induced osteosarcoma cells apoptosis by p53 stabilization. Life Sci 208:232-238 (2018). PubMed: 30005830
      View all Publications for this product

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      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-MDM2 [SMP 14] antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      20 µg
      Gel Running Conditions
      Reduced Denaturing (8% gel)
      Sample
      Human Cell lysate - whole cell (human medulloblastoma cells)
      Specification
      human medulloblastoma cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      提交于 Apr 18 2014

      Western blot abreview for Anti-MDM2 antibody [SMP 14]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (pancreas)
      Loading amount
      40 µg
      Specification
      pancreas
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      提交于 Oct 10 2011

      Western blot abreview for Anti-MDM2 antibody [SMP 14]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (lungs)
      Loading amount
      40 µg
      Specification
      lungs
      Gel Running Conditions
      Reduced Denaturing (8%)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      提交于 Jul 01 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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