Anti-MDM2抗体- ChIP Grade (ab226939)
Key features and details
- Rabbit polyclonal to MDM2 - ChIP Grade
- Suitable for: WB, IP, IHC-P, ChIP
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MDM2抗体- ChIP Grade
参阅全部 MDM2 一抗 -
描述
兔多克隆抗体to MDM2 - ChIP Grade -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ChIPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Cat, Dog, Pig, Rhesus monkey -
免疫原
Recombinant fragment within Human MDM2 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q00987-1 -
阳性对照
- WB: Neuro-2a, GL261, C8D30, NIH/3T3, RAW 264.7, BCL-1, C2C12, Jurkat, Raji and NCIH-929 whole cell extracts; MDM2 transfected HEK-293T whole cell extract; HepG2, starved for 16 hours, whole cell extract. IP: Jurkat whole cell extract. IHC-P: Human lung and lung cancer tissues. ChIP: PC-3 chromatin extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226939于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
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IP |
1/100 - 1/500.
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IHC-P | (1) |
1/100 - 1/1000.
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ChIP |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
IP
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. |
ChIP
Use at an assay dependent concentration. |
靶标
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功能
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. -
组织特异性
Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues. -
疾病相关
Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding. -
序列相似性
Belongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain. -
结构域
Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself. -
翻译后修饰
Phosphorylated in response to ionizing radiation in an ATM-dependent manner.
Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiquitinated by USP7; leading to stabilize it. -
细胞定位
Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 493939 Cat
- Entrez Gene: 540378 Cow
- Entrez Gene: 403693 Dog
- Entrez Gene: 4193 Human
- Entrez Gene: 17246 Mouse
- Entrez Gene: 100125959 Pig
- Entrez Gene: 718284 Rhesus monkey
- Omim: 164785 Human
see all -
别名
- ACTFS antibody
- Double minute 2 protein antibody
- E3 ubiquitin-protein ligase Mdm2 antibody
see all
图片
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All lanes : Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : MDM2 transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
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MDM2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 4 µg ab226939. Western blot was performed from the immunoprecipitate using ab226939 at 1/500 dilution.
Lane 1: Jurkat whole cell extract 30 μg (Input).
Lane 2: Control IP in Jurkat whole cell extract with 4 μg of preimmune Rabbit IgG.
Lane 3: ab226939 IP in Jurkat whole cell extract.
7.5 % SDS-PAGE gel.
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All lanes : Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NHI-H929 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : GL261 whole cell extract
Lane 3 : C8D30 whole cell extract
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 5 : BCL-1 whole cell extract
Lane 6 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 7 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded human lung cancer tissue stained for MDM2 with ab226939 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human lung tissue stained for MDM2 with ab226939 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/3000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line), starved for 16 hours, whole cell extract
Lane 2 : HepG2, starved for 16 hours and treated with 1 uM staurosporine for 24 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
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Cross-linked ChIP was performed with PC-3 (human prostate adenocarcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab226939. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab226939 被引用在 4 文献中.
- Sharma BK et al. Fibrinogen activates focal adhesion kinase (FAK) promoting colorectal adenocarcinoma growth. J Thromb Haemost 19:2480-2494 (2021). PubMed: 34192410
- Wang Y et al. MDM2 promotes the proliferation and inhibits the apoptosis of pituitary adenoma cells by directly interacting with p53. Endokrynol Pol 71:425-431 (2020). PubMed: 32797477
- Cheng Z et al. K-Ras-ERK1/2 accelerates lung cancer cell development via mediating H3K18ac through the MDM2-GCN5-SIRT7 axis. Pharm Biol 57:701-709 (2019). PubMed: 31613681
- He P et al. UBA2 promotes proliferation of colorectal cancer. Mol Med Rep 18:5552-5562 (2018). PubMed: 30387828