Anti-MDC1抗体[MDC1-50] (ab50003)
Key features and details
- Mouse monoclonal [MDC1-50] to MDC1
- Suitable for: ICC, IP, ICC/IF, WB
- Reacts with: Human, Monkey
- Isotype: IgG2a
概述
-
产品名称
Anti-MDC1抗体[MDC1-50]
参阅全部 MDC1 一抗 -
描述
小鼠单克隆抗体[MDC1-50] to MDC1 -
宿主
Mouse -
经测试应用
适用于: ICC, IP, ICC/IF, WBmore details -
种属反应性
与反应: Human, Monkey -
免疫原
Recombinant fragment, corresponding to amino acids 2-200 of Human MDC1
-
阳性对照
- WB: total cell extract of G361 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
-
纯化说明
This antibody is a purified Mouse Immunoglobulin. -
克隆
单克隆 -
克隆编号
MDC1-50 -
骨髓瘤
NS1 -
同种型
IgG2a -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50003于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
|
ICC/IF | (3) |
Use at an assay dependent concentration.
|
WB |
Use at an assay dependent concentration.
|
说明 |
---|
ICC
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
靶标
-
功能
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. -
组织特异性
Highly expressed in testis. -
序列相似性
Contains 2 BRCT domains.
Contains 1 FHA domain. -
结构域
Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX. -
翻译后修饰
Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. -
细胞定位
Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks. - Information by UniProt
-
数据库链接
- Entrez Gene: 9656 Human
- GenBank: NP_055456 Human
- Omim: 607593 Human
- SwissProt: Q14676 Human
- Unigene: 653495 Human
-
别名
- Homologue to Drosophila photoreceptor protein calphotin antibody
- MDC 1 antibody
- Mdc1 antibody
see all
数据表及文件
-
SDS download
-
Datasheet download
文献 (15)
ab50003 被引用在 15 文献中.
- Lee KY & Dutta A Chk1 promotes non-homologous end joining in G1 through direct phosphorylation of ASF1A. Cell Rep 34:108680 (2021). PubMed: 33503415
- Ka NL et al. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer. Cell Rep 37:110138 (2021). PubMed: 34936865
- Bergstrand S et al. Biallelic mutations in WRAP53 result in dysfunctional telomeres, Cajal bodies and DNA repair, thereby causing Hoyeraal-Hreidarsson syndrome. Cell Death Dis 11:238 (2020). PubMed: 32303682
- Mooser C et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317
- Singh N et al. MDC1 depletion promotes cisplatin induced cell death in cervical cancer cells. BMC Res Notes 13:146 (2020). PubMed: 32160908