重组Anti-CSF-1-R抗体[SP211] (ab183316)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP211] to CSF-1-R
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CSF-1-R抗体[SP211]
参阅全部 CSF-1-R 一抗 -
描述
兔单克隆抗体[SP211] to CSF-1-R -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-Pmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human colon, lung, liver, skin, tonsil and skin squamous cell carcinoma, rat spleen tissues. Flow Cyt (intra): SK-BR-3 cells.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP211 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183316于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/20.
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IHC-P | (3) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. |
说明 |
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Flow Cyt (Intra)
1/20. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. |
靶标
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功能
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
组织特异性
Expressed in bone marrow and in differentiated blood mononuclear cells. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1436 Human
- Entrez Gene: 12978 Mouse
- Entrez Gene: 307403 Rat
- Omim: 164770 Human
- SwissProt: P07333 Human
- SwissProt: P09581 Mouse
- SwissProt: Q00495 Rat
- Unigene: 586219 Human
see all -
别名
- C FMS antibody
- CD 115 antibody
- CD115 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling CSF-1-R with ab183316 at 1/100 dilution (1.13 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183316 for 10 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CSF-1-R with ab183316 at 1/100 dilution (1.13 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183316 for 10 mins at room temperature. -
Intracellular Flow Cytometry analysis of SK-BR-3 (human mammary gland adenocarcinoma) labeling CSF-1-R with purified ab183316 at 1/20 dilution (5.65µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).
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Immunohistochemical analysis of human tonsil tissue labeling CSF-1-R using ab183316 at a 1/100 dilution
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Immunohistochemical analysis of human skin tissue labeling CSF-1-R using ab183316 at a 1/100 dilution
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Immunohistochemical analysis of human skin squamous cell carcinoma tissue labeling CSF-1-R using ab183316 at a 1/100 dilution
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Immunohistochemical analysis of human colon tissue labeling CSF-1-R using ab183316 at a 1/100 dilution.
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Immunohistochemical analysis of human lung tissue labeling CSF-1-R using ab183316 at a 1/100 dilution.
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Immunohistochemical analysis of human liver tissue labeling CSF-1-R using ab183316 at a 1/100 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab183316 被引用在 16 文献中.
- Sinha M et al. Modulation of myeloid and T cells in vivo by Bruton's tyrosine kinase inhibitor ibrutinib in patients with metastatic pancreatic ductal adenocarcinoma. J Immunother Cancer 11:N/A (2023). PubMed: 36593070
- Johnson BE et al. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer. Cell Rep Med 3:100525 (2022). PubMed: 35243422
- Xiaocui L et al. CSF-1-induced DC-SIGN+ macrophages are present in the ovarian endometriosis. Reprod Biol Endocrinol 20:48 (2022). PubMed: 35260161
- Arnoletti JP et al. Pancreatic Ductal Adenocarcinoma (PDAC) circulating tumor cells influence myeloid cell differentiation to support their survival and immunoresistance in portal vein circulation. PLoS One 17:e0265725 (2022). PubMed: 35316296
- Sundaram S et al. Deciphering the Immune Complexity in Esophageal Adenocarcinoma and Pre-Cancerous Lesions With Sequential Multiplex Immunohistochemistry and Sparse Subspace Clustering Approach. Front Immunol 13:874255 (2022). PubMed: 35663986